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中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (7): 738-742.

• 基础研究 • 上一篇    下一篇

姜黄素对肝肺综合征大鼠的保护作用

李旭炯1,刘 安2,陈云霞3,李淑芬1   

  1. 1 长治医学院生理学教研室,长治 046000,山西;2 中国中医科学院中药研究所,北京 100700;3 长治医学院微生物学教研室,长治 046000,山西
  • 收稿日期:2016-08-03 修回日期:2016-11-07 出版日期:2017-07-26 发布日期:2017-07-19
  • 作者简介:李旭炯,男,副教授,主要从事肝脏病变及其并发症的研究。 E-mail:qazw3000@126.com
  • 基金资助:

    科技部重大新药创制重大专项(2014ZX09304307-001);长治医学院科技创新团队项目(CX201509)

Curcumin alleviate intrapulmonary inflammation in rat experimental hepatopulmonary syndrome

LI Xujiong 1, LIU An 2, CHEN Yunxia 3, LI Shufen 1   

  1. 1 Physiology Department of Changzhi Medical College, Changzhi 046000, Shanxi, China; 2 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 3 Microbiology Department of Changzhi Medica l College, Changzhi 046000, Shanxi, China
  • Received:2016-08-03 Revised:2016-11-07 Online:2017-07-26 Published:2017-07-19

摘要:

目的: 观察姜黄素对胆总管结扎诱导的大鼠肝肺综合征(HPS)肺组织炎性损伤的保护作用。方法: 造模1周后,姜黄素(50 mg·kg-1·d-1)通过灌胃给予。给药2周后,进行血气分析、血浆TNF-α及肝功能检测。对肺、肝组织病理改变(HE染色)进行观察。并采用免疫荧光染色对肺组织CD68、NF-κB P-65及TNF-α进行检测,同时采用Western blotting法对IκBα/p-IκBα进行检测。结果: 姜黄素减轻HPS大鼠的肺组织巨噬细胞聚集及炎性损伤、降低了AaPO2,同时也降低了HPS大鼠血浆和肺组织TNF-α含量及NF-κB激活程度。结论: 姜黄素对HPS大鼠的肺组织炎性损伤具有保护作用,其机制可能与NF-κB激活程度减轻有关。

关键词: 肝肺综合征, 姜黄素, TNF-α, NF-κB

Abstract:

AIM: To evaluate the protective effect of curcumin in an experimental model of hepatopulmonary syndrome (HPS)  induced by bile duct ligation in rats.  METHODS: One week after surgery, curcumin (50 mg·kg-1·d-1) was administered intragastrically for two weeks. Arterial blood gas analysis was performed. Plasma TNF-α and ALT level was measured. Pathological change in rat lung tissue and liver tissue were observed through HE staining. CD68, TNF-α and NF-κB P-65 were detected by immunofluorescence, and IκBα/p-IκBα was assessed by Western blotting. RESULTS: Curcumin alleviated lung tissue and pulmonary intravascular monocyte accumulation and significantly decreased AaPO. Also, pulmonary TNF-α level and lung tissue NF-κB activity were reduced. CONCLUSION: Curcumin can protect HPS rat from pulmonary inflammation injury. The mechanism may be related with anti-inflammation by inhibition of TNF-α/NF-κB.

Key words: hepatopulmonary syndrome, curcumin, TNF-α, NF-κB

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