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中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (12): 1335-1339.doi: 10.12092/j.issn.1009-2501.2018.12.003

• 基础研究 • 上一篇    下一篇

阿魏酸对急性肝损伤的保护作用

钟正灵1,张 飞2,江玉华2,张炜婷3,童九翠4,李 娟1,储冀汝1,谢海棠4   

  1. 1皖南医学院第一附属医院弋矶山医院《中国临床药理学与治疗学》编辑部; 2皖南医学院2014级药学本科;3皖南医学院2016级临床医学; 4皖南医学院第一附属医院弋矶山医院药物临床评价中心,芜湖 241001,安徽
  • 收稿日期:2018-12-10 修回日期:2018-08-08 出版日期:2018-12-26 发布日期:2018-12-27
  • 通讯作者: 谢海棠,女,博士,硕士生导师,教授,研究方向:药物代谢动力学及药效学。 E-mail:294426443@qq.com
  • 作者简介:钟正灵,女,硕士,讲师,研究方向:中药药理。 E-mail:517541595@qq.com
  • 基金资助:

    皖南医学院中青年基金(WK201004F);皖南医学院重点科研项目培育基金(WK2014ZF05)

Protective effects of Ferulic acid on acute liver injury induced by D-GalN/LPS in mice

ZHONG Zhengling1,ZHANG Fei2,JIANG Yuhua2,ZHANG Weiting3,TONG Jiucui4,LI Juan1,CHU Jiru1,XIE Haitang4   

  1. 1 Editorial Department of Chinese Clinical Pharmacology and Therapeutics, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical College;2 Undergraduate of Pharmaceutical Science, Grade 2014, Wannan Medical College;3 Grade 2016 Clinical Medicine of Wannan Medical College;4 Clinical Drug Evaluation Center,Yijishan Hospital, the First Affiliated Hospital of Wannan Medical College, Wuhu 241001,Anhui,China
  • Received:2018-12-10 Revised:2018-08-08 Online:2018-12-26 Published:2018-12-27

摘要:

目的: 研究阿魏酸对D-半乳糖胺/脂多糖诱导小鼠急性肝损伤的保护作用。方法: 60只BALB/c小鼠,随机分成正常对照组、D-半乳糖胺/脂多糖模型组(600 mg·30 μg/kg)、联苯双酯组(200 mg/kg)、阿魏酸高、中、低剂量组(30、15、7.5 mg/kg),每组10只。连续灌胃给药21 d,每天一次,末次给药2 h后处死小鼠,取新鲜肝组织,眼球采血,测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及肝组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量;观察小鼠肝组织的病理变化;称量小鼠体质量,计算小鼠肝指数。结果: 与D-半乳糖胺/脂多糖模型组比较,联苯双酯组和阿魏酸高、中、低剂量组小鼠肝脏指数、血清ALT、AST水平不同程度降低(P<0.05);肝组织中SOD、GSH-Px活性不同程度升高(P<0.05),MDA 水平不同程度降低(P<0.05); 肝脏病理学检查显示,联苯双酯组和阿魏酸高、中、低剂量组均能不同程度地减轻肝细胞变性、坏死。结论: 阿魏酸对D-半乳糖胺/脂多糖诱导的急性肝损伤具有一定的保护作用,其机制可能与抗氧化作用相关联。

关键词: 阿魏酸, 脂多糖, 急性肝损伤, 抗氧化

Abstract:

AIM:To study the protective effects of Ferulic acid on acute liver injury induced by D-GalN/LPS in mice. METHODS:A total of 60 BALB/c mice were divided into six groups:control group,D-galactosamine/lipopolysaccharide(D-GalN/LPS) model group(600 mg·30 μg/kg),bifendate group(200 mg/kg),the high,middle and low dose groups of Ferulic acid (30, 15, 7.5 mg/kg, respectively), with 10 mice in each group. All groups received intragastric administration once a day for 21 days. Mice were sacrificed 2 h after the last administration to collect liver tissue and blood from eyeball, and ALT, AST, SOD, GSH-Px, MDA were detected. The pathological changes of liver tissue were observed, and liver index was calculated according to the body weight of mice.RESULTS:Compared with D-GalN/LPS model group, bifendate group(200 mg/kg) and the high, middle and low dose groups of Ferulic acid presented siginificant decrease of the liver index, serum ALT, AST and MDA (P<0.05), and significant increase of SOD and GSH-Px (P<0.05). Pathological analysis of the liver tissue showed that bifendate and different doses of Ferulic acid could alleviate the hepatocytosis and necrosis. CONCLUSION: Ferulic acid has protective effect on acute liver injury induced by D-GalN/LPS in mice. The mechanism maybe related to the anti-oxidation effect of Ferulic acid.

Key words: Ferulic acid, lipopolysaccharide, acute liver injury, anti-oxidation

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