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中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (7): 737-743.doi: 10.12092/j.issn.1009-2501.2019.07.003

• 基础研究 • 上一篇    下一篇

姜黄素对高血压病大鼠全脑缺血再灌注时海马神经元损伤及皮质酮、SGK1表达的影响

俞陈陈1,刘树群1,刘建龙1,曹 红2,刘雪娇1,蔡茜茜1   

  1. 1温州医科大学附属第一医院麻醉科,2温州医科大学附属第二医院麻醉科,温州 325015,浙江
  • 收稿日期:2018-12-27 修回日期:2019-06-20 出版日期:2019-07-26 发布日期:2019-07-29
  • 通讯作者: 曹红,女,硕士,教授,研究方向:围术期重要器官保护。 Tel:13758497562 E-mail: caohongwz@163.com
  • 作者简介:俞陈陈,女,硕士,医师,研究方向:围术期重要器官保护。 Tel:18267737578 E-mail:57298871@qq.com
  • 基金资助:

    国家自然科学基金资助项目(81073125);浙江省科技厅资助项目(2011C23022)

Effects of curcumin on injury in hippocampal neurons and expression of blood corticosterone and SGK1 in hippocampal during global cerebral ischemia-reperfusion injury in hypertensive rats

YU Chenchen1, LIU Shuqun1, LIU Jianlong1, CAO Hong2, LIU Xuejiao1, CAI Xixi1   

  1. 1 Department of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China; 2 Department of Anesthesiology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, China
  • Received:2018-12-27 Revised:2019-06-20 Online:2019-07-26 Published:2019-07-29

摘要:

目的:探讨姜黄素对高血压病大鼠全脑缺血再灌注时海马神经元损伤及血清皮质酮、海马血清和糖皮质激素诱导激酶1(serum-and glucocorticoid-inducible kinases1,SGK1)表达的影响。方法:雄性Wistar-Kyoto(WKY)大鼠和自发性高血压病(SH)大鼠,随机分为5组:假手术组(W-Sham、S-Sham)、缺血再灌注组(W-IR、S-IR)和姜黄素组(S-Cur)。各组按再灌注时间分为3 h、12 h、1 d、3 d和7 d 5个时间点。采用四血管阻断法制备全脑缺血再灌注模型(缺血10 min后恢复灌注),HE染色观察海马CA1区神经细胞形态,Nissl染色计数海马CA1区平均锥体细胞密度,ELISA法检测血清皮质酮及海马SGK1,于再灌注后7 d观察行为学。结果:与假手术组比较,缺血再灌注组学习和记忆能力下降,海马CA1区神经元损伤加重,血清皮质酮水平增加,海马SGK1表达上调(P<0.05);与W-IR组比较,S-IR组学习和记忆能力下降,海马CA1区神经元损伤加重,血清皮质酮水平增加(P<0.05),海马SGK1蛋白表达下调(P<0.05);姜黄素组学习和记忆能力明显改善,海马CA1区神经元损伤减轻,血清皮质酮水平下降(P<0.05),海马SGK1蛋白表达上调(P<0.05)。结论:姜黄素减轻高血压病大鼠全脑缺血再灌注海马神经元损伤的机制可能与抑制皮质酮、上调SGK1表达有关。

关键词: 姜黄素, 全脑缺血再灌注, 认知功能, 皮质酮, 血清和糖皮质激素诱导激酶1

Abstract:

AIM: To investigate the effects of curcumin on injury in hippocampal neurons and expression of blood corticosterone and serum-and glucocorticoid-inducible kinases1(SGK1) in hippocampal during global cerebral ischemia-reperfusion injury in hypertensive rats. METHODS: Male Wistar-Kyoto rats and male spontaneously hypertensive rats were randomLy divided into five groups (n=30): sham group (W-Sham and S-Sham), ischemia-reperfusion group (W-IR and S-IR), curcumin group (S-Cur). Rats were sacrificed at 3 h, 12 h, 1 d, 3 d, 7 d after reperfusion. Global brain ischemic model was established by 4-VO method. HE Staining was used to observe the vertebral cell morphology in hippocampal CA1 region. Nissl Stainning was applied to detect the average density of vertebral cell in hippocampal CA1 region. The corticosterone in blood and SGK1 in hippocampal were determined by ELISA. At 7d after reperfusion the behavior of the rats was observed. RESULTS:Compared with sham group , the ability to learn and remember was greatly decreased in ischemia-reperfusion group, the number of injured neurons was significantly enhanced , the corticosterone in blood was extremely enhanced(P<0.05), the expression of SGK1 in hippocampal was significantly increased(P<0.05). Compared with W-IR, the ability of S-IR to learn and remember was greatly decreased, the number of injured neurons was extremely enhanced, the corticosterone in blood was significantly increased(P<0.05), the expression of SGK1 in hippocampal was significantly decreased(P<0.05). The number of injured neurons was greatly declined in S-Cur, the ability to learn and remember was improved , the corticosterone in blood was extremely decreased(P<0.05), the expression of SGK1 in hippocampal was significantly increased(P<0.05). CONCLUSION: Inhibition of corticosterone in blood and overexpression of SGK1 in hippocampus may be involved in the mechanism by which curcumin reduces global cerebral IR injuty in hypertensive rats.

Key words: curcumin, cerebral ischemia/reperfusion, cognitive function, corticosterone, serum-and glucocorticoid-inducible kinases1

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