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中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (7): 786-791.doi: 10.12092/j.issn.1009-2501.2019.07.010

• 基础研究 • 上一篇    下一篇

萝卜硫素介导Akt/p70S6K信号传导诱导人鼻咽癌CNE-2细胞凋亡

赵 丽,华 夏,谭 晔,胡承莲   

  1. 恩施土家族苗族自治州中心医院急诊科,恩施 445000,湖北
  • 收稿日期:2018-12-18 修回日期:2019-06-08 出版日期:2019-07-26 发布日期:2019-07-29
  • 通讯作者: 胡承莲,女,本科,主治医师,主要研究鼻咽部疾病。 E-mail: hubei8888xx@163.com
  • 作者简介:赵丽,女,本科,主治医师,主要研究肿瘤疾病的预防与治疗。 Tel: 18695050606 E-mail: 307312548@qq.com

Effects of sulforaphane-induced Akt/p70S6K signal transduction pathway on apoptosis of human nasopharyngeal cancer cells CNE-2

ZHAO Li, HUA Xia, TAN Ye, HU Chenglian   

  1. Department of Emergency, Central Hospital of En-shi Autonomous Prefecture, Enshi 445000, Hubei, China
  • Received:2018-12-18 Revised:2019-06-08 Online:2019-07-26 Published:2019-07-29

摘要:

目的:研究萝卜硫素对人鼻咽癌CNE-2细胞增殖、凋亡的影响及其对Akt/p70S6K信号传导的作用。方法:采用CCK-8实验分析萝卜硫素对CNE-2细胞生长增殖的影响,流式细胞仪检测萝卜硫素对CNE-2细胞凋亡率及细胞周期分布的影响,Western blot技术检测CNE-2细胞中调控细胞周期的相关蛋白及Akt/p70S6K信号通路关键蛋白的表达水平。结果:采用萝卜硫素干预CNE-2细胞后,细胞增殖抑制率及凋亡率相对于对照组呈浓度依赖的方式显著增高,差异有统计学意义(P<0.05);流式细胞仪分析提示,萝卜硫素干预能够将细胞停留在S期及G2/M期,并且萝卜硫素还能抑制细胞周期蛋白Cyclin A、Cyclin B、Cyclin D、Cyclin E以及CDK/p34的表达水平,与对照组相比差异有统计学意义(P<0.05);此外,萝卜硫素还能抑制Akt/p70S6K信号通路关键蛋白p-Akt及p70S6K蛋白表达水平(P<0.05),但萝卜硫素与Akt激动剂IGF-I共同处理细胞后,上述蛋白表达量与对照组相比没有统计学差异(P>0.05)。结论:萝卜硫素可能通过干扰CNE-2细胞中Akt/p70S6K信号传导而发挥有效的抗增殖及促凋亡的作用。

关键词: 萝卜硫素, 人鼻咽癌细胞, 增殖, 凋亡, Akt/p70S6K信号通路

Abstract:

AIM: To study the effect of sulforaphane on the proliferation and apoptosis of CNE-2 cells, and to investigate the role of sulforaphane in the Akt/p70S6K signaling pathway.  METHODS: CCK-8 test was used to detect cellular growth of CNE-2. Flow cytometry was carried out to observe apoptosis and cell cycle status. Expression levels of several cell cycle associated proteins and Akt/p70S6K pathway related proteins were determined by Western blot.RESULTS:Cell proliferation inhibitory rates and apoptotic rates of CNE-2 cells in various doses of sulforaphane groups were significantly increased as compared with control group with a dose-dependent manner (P<0.05). Flow cytometry results showed that sulforaphane arrestted NPC cells at the S-phases and G2/M-phases, and the expression levels of Cyclin A, Cyclin B, Cyclin D, Cyclin E and CDK/p34 were greatly decreased in sulforaphane group as compared with control group (P<0.05). Moreover, the expression levels of p-Akt and p70S6K in sulforaphane group were also decreased as compared with control group (P<0.05). However, the expression levels of these proteins above in sulforaphane+ IGF-I group unchanged as compared with control group (P>0.05). CONCLUSION: Sulforaphane exerts anti-proliferative and pro-apoptotic effects on CNE-2 cells through interfering with the Akt/p70S6K signaling pathways.

Key words: sulforaphane, human nasopharyngeal cancer cells, proliferation, apoptosis, Akt/p70S6K signaling pathways

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