中国儿童保健杂志 ›› 2015, Vol. 23 ›› Issue (8): 817-820.DOI: 10.11852/zgetbjzz2015-23-08-11

• ·基础科研论著· • 上一篇    下一篇

丙酮酸乙酯对宫内感染致脑损伤新生仔鼠12-脂氧合酶表达和细胞凋亡的影响

孔祥颖1,李晓捷1,宋福祥1,Rajesh2   

  1. 1 佳木斯大学康复医学院/佳木斯大学附属第三医院/佳木斯大学儿童神经康复实验室,黑龙江 佳木斯 154003;
    2 佳木斯大学留学生学院,黑龙江 佳木斯 154003
  • 收稿日期:2014-12-15 出版日期:2015-08-10 发布日期:2015-08-10
  • 通讯作者: 李晓捷,E-mail:xiaojljms@vip.163.com
  • 作者简介:孔祥颖 (1983-),女,黑龙江人,讲师,硕士研究生,主要研究方向为小儿脑损伤发病机制及早期防治研究。

Protective effects of ethyl pyruvate on expressions of 12-lipaxygenase and apoptosis on rat's offspring's damaged brain caused by intrauterine infection.

KONG Xiang-ying1,LI Xiao-jie1,SONG Fu-xiang1,Rajesh2.   

  1. (1 Rehabilitation Medicine College of Jiamusi University,Third Affiliated Hospital of Jiamusi University,Children Neural Rehabilitation Laboratory of Jiamusi University,Jiamusi,Heilongjiang 154003,China; 2 College of Foreign Students Education of Jiamusi University,Jiamusi,Heilongjiang 154003,China)
    Corresponding author:LI Xiao-jie,E-mail:xiaojljms@vip.163.com
  • Received:2014-12-15 Online:2015-08-10 Published:2015-08-10
  • Contact: LI Xiao-jie,E-mail:xiaojljms@vip.163.com

摘要: 目的 研究丙酮酸乙酯(ethyl pyruvate,EP)对宫内感染致新生仔鼠脑损伤12-脂氧合酶表达和细胞凋亡的影响。方法 36只孕鼠随机分为LPS组、EP组、NS组,每组各12只。LPS组:于孕17天、18天孕鼠连续两天腹腔注射脂多糖380 μg/kg;EP组:相同的方法制备孕鼠宫内感染模型,并于孕鼠腹腔注射LPS后立即给予腹腔注射EP 40 mg/kg;NS组:给予等量生理盐水腹腔注射。孕鼠分娩后,取胎盘进行HE染色,以观察宫内感染情况。剔除早产鼠,随机选取各组仔鼠36只,分别于出生后1 d、3 d、7 d进行检测,观察脑组织12-脂氧合酶(12-lipoxygenase,12-LOX)的表达和神经细胞凋亡情况。结果 与NS组比较,LPS组孕鼠胎盘组织可见明显的炎性细胞浸润,血管充血等病理改变;与LPS组相比较,EP组仔鼠出生后1、3、7 d脑组织12-LOX的表达量均低于LPS组(P<0.05)、神经细胞凋亡指数均也均低于LPS组(P<0.05)。结论 EP对宫内感染致脑损伤新生仔鼠的脑组织具有保护作用,可能与12-LOX表达水平和细胞凋亡降低相关。

关键词: 丙酮酸乙酯, 宫内感染, 脑损伤, 12-脂氧合酶, 细胞凋亡
中图分类号:R722 文献标识码:A 文章编号:1008-6579(2015)08-0817-04 doi:10.11852/zgetbjzz2015-23-08-11

Abstract: Objective To study the effect of ethyl pyruvate(EP) in the expression of 12-lipoxygenase(12-LOX) and apoptosis changes on rat's offspring's damaged brain caused by intrauterine infection. Methods Pregnant wistar rats of lipopolysaccharide(LPS) group were consecutively injected with lipopolysaccharide [380 μg/(kg·day)] intraperitoneally.Pregnant Wistar rats of EP group were consecutively injected with LPS intraperitoneally,and EP was administrated intraperitoneally at a dose of 40 mg/kg immediately.12 pregnant rats were injected with normal saline intraperitoneally as control group.After the delivery of pups,thirty-six pups were selected in every group randomly.placenta from rats of LPS group was taken for hematoxylline-eosin (HE) staining to confirm its intrauterine infection.12-LOX expression in brain tissue was studied through immunohistochemical and western-blot analysis.TUNEL was used to observe the pathological changes of the brain tissue. Results Obvious pathological changes were observed in the placenta in the infection groups.The expression of apoptosis was lower in EP group and NS group than in the LPS group (P<0.05),and lower in NS group than that in the EP group (P<0.05).The expression of 12-lipoxygenase was lower in EP group and NS group than that in the LPS group (P<0.05),and lower in NS group than that in the EP group (P<0.05). Conclusion EP confers potent protection against LPS-induced brain injury via significantly reduced the expression of 12-LOX and anti-apoptotic properties.

Key words: ethyl pyruvate, intrauterine infection, brain injury, 12-lipoxygenase, apoptosis

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