中国儿童保健杂志 ›› 2016, Vol. 24 ›› Issue (9): 928-930.DOI: 10.11852/zgetbjzz2016-24-09-10

• 基础科研论著 • 上一篇    下一篇

骨髓间充质干细胞移植对Tourette综合征大鼠纹状体脑源性神经营养因子表达的影响

刘秀梅1, 王学明2, 衣明纪3   

  1. 1 烟台毓璜顶医院儿科,山东 烟台 264000;
    2 烟台毓璜顶医院烧伤整形美容科,山东 烟台 264000;
    3 青岛大学医学院附属医院儿童保健科,山东 青岛 266021
  • 收稿日期:2016-02-29 出版日期:2016-09-10 发布日期:2016-09-10
  • 作者简介:刘秀梅(1979-),女,山东人,副主任医师,博士学位,主要研究方向为儿童保健及发育行为儿科学。
  • 基金资助:
    国家自然科学基金(81101017);山东省优秀中青年科学家科研奖励基金(BS2010F030);烟台市科学技术发展计划项目(2010148-24)

Influence of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome.

LIU Xiu-mei1, WANG Xue-ming2, YI Ming-ji3   

  1. 1 Pediatric Department;
    2 Burn Plastic Surgery Department,Yantai Yuhuangding Hospital,Yantai,Shandong 264000,China;
    3 Child Health Care Department of Affiliated Hospital of Medical College Qingdao University,Yantai,Shandong 266021,China
  • Received:2016-02-29 Online:2016-09-10 Published:2016-09-10

摘要: 目的 考察骨髓间充质干细胞(MSC)移植对Tourette综合征(TS)大鼠纹状体内脑源性神经营养因子(BDNF)表达的影响,探讨MSC移植治疗TS的作用机制。方法 采用立体定向技术向大鼠纹状体内微量灌注抗基底神经节抗体(ABGA),制备自身免疫TS动物模型;采用密度梯度离心法在体外分离、培养和扩增大鼠MSC,经流式细胞仪鉴定后将MSC标记并移植到TS大鼠纹状体内。通过ELISA及RT-PCR技术检测大鼠脑内BDNF水平。结果 将MSC移植到TS大鼠纹状体内,自移植后7 d开始观察到TS大鼠的刻板行为减少。TS大鼠纹状体内BDNF水平升高(P<0.05);MSC移植后TS大鼠纹状体内BDNF含量下降。结论 MSC移植能减少TS大鼠的刻板行为、降低TS大鼠体内的BDNF含量。

关键词: 骨髓间充质干细胞, 移植, Tourette综合征, 脑源性神经营养因子

Abstract: Objective To investigate bone marrow mesenchymal stem cells (MSC) transplantation on brain-derived neurotrophic factor (BDNF) expression in the striatum of Tourette syndrome (TS) rat,and to explore the possible mechanism of MSC transplantation in the treatment of TS. Methods Seventy-two Wistar rats were randomly allocated to the control group and the two experimental groups,namely,TS rats+vehicle and TS rats+MSC.MSCs were co-cultured with 5-bromodeoxyuridine (BrdU) for 24 h for labeling prior to grafting.An autoimmune TS animal model was adopted in the present study.Rat MSCs were cultured and expanded using density gradient centrifugation in vitro,identified by flow cytometry and transplanted into the striatums of TS animal.BDNF level was detected by ELISA and RT-PCR techniques. Results Stereotypic behaviors reduced in TS rats 7 days after MSC transplantation into rat striatum.BDNF level in striatum of TS rat increased (P<0.05).After MSC transplantation,the BDNF level decreased in the striatum of TS rat. Conclusions Intrastriatal transplantation of MSCs can provide relief from the stereotypic behavior of TS.BDNF level reduces in TS rats after MSC transplantation.

Key words: bone marrow mesenchymal stem cells, transplantation, Tourette syndrome, brain-derived neurotrophic factor

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