关键词: 脑瘫, 疗效, 人脐血间充质干细胞, 侧脑室内移植, 鼠神经生长因子

Abstract: Objective To study the treatment effect of human umbilical cord blood mesenchymal stem cells(hUCBMSCs) combined with mouse nerve growth factor (mNGF) to the cerebral palsy rats through lateral intraventricular transplantation. Methods 100 ml cord blood from full-term newborn was collected and to separate,culture and purify hUCBMSCs then detect and identify them.100 seven-day-old SD rats were randomly divided into 10 normal group and 90 experiment group(cerebral palsy produced by ischemia and hypoxia ) while the experiment group were split into cerebral palsy (CP) model group,phosphate buffer solution (PBS) transplantation group,mNGF transplantation group,human umbilical cord blood-derived mesenchymal stem cells transplantation group,and human umbilical cord blood-derived mesenchymal stem cells combined with mouse nerve growth factor transplantation group.After 1 w modeling success MSCs was injected,mNGF and PBS into Lateral ventricles.After transplantation in 1 w,2 w and 3 w,the survival and migration of MSCs with 5 bromine -2 (BrdU) labeled were observed.In the fourth week,the neural behavior ability of rats were studied and the rat brain tissues were taken to detect the expression of its GFAP and NSE. Results 1)The survival,migration of hUCBMSCs mainly distributed at the site of the left lateral ventricle,frontal lobe,parietal cortex and white matter.Along with the time going,its migration was to the surrounding brain tissue.BrdU,NSE,GFAP positive cells decreased gradually,and the difference was statistically significant (P<0.05).Compared with model group and sham transplantation,the footprints latency and maze escape latency of single transplantation groups and combined transplantation group were shorten,footprints repeat spacing and foot slippage number decreasing as well,and transplantation group was better than that of single transplantation groups,the difference was significant (P<0.05);2)HE staining:the cell morphology and brain tissue damage in the single transplantation group were better than that of the cerebral palsy group,and the recovery of the combined transplantation group was better than that of the single tran-splantation group. Conclusion Through intraventricular transplantation,exogenous MSCs transplantation combined with mNGF can improve the long-term behavior and brain damage in cerebral palsy rats.

Key words: cerebral palsy, efficacy, human umbilical cord blood mesenchymal stem cells, lateral intraventricular transplantation, mouse nerve growth factor