关键词: 支气管哮喘, ADAM33基因, V4位点, 维生素D, 乌鲁木齐, 儿童

Abstract: Objective To explore correlation of ADAM33 V4 gene polymorphism and vitamin D (Vit D) level with asthma of children in Urumqi. Method Totally 154 asthmatic children and 120 healthy non-asthmatic children aged 3~15 in Urumqi were selected.ADAM 33 V4 gene single nucleotide polymorphism loci (SNPs) genotyping were detected using PCR polymerase chain reaction and VitD levels were detected by ELISA.Further stratified according ethnic groups were performed and correlation of ADAM33 V4 gene polymorphism and VitD level with asthma were analyzed. Result 1)V4 sites of the frequency distribution of three genotypes between cases and control groups were statistically significant differences (χ²=6.806,P=0.033) and,it showed no significant difference among the different ethnic groups (case group:χ²=0.714,P=0.699;control group:χ²=5.898,P=0.052).2)VitD levels in the case group and the control group were statistically different(t=2.341,P=0.020). Conclusions 1) ADAM33 V4 gene loci is correlated with childhood asthma morbidity in children in Urumqi,but there is no difference among nationalities.V4 site after the G point mutation may be a risk factor of bronchial asthma.2) VitD levels is correlated with childhood asthma morbidity in Urumqi.

Key words: asthma, ADAM33, V4 loci polymorphism, vitamin D, Urumqi, children