关键词: microRNA 20a, 小儿肺炎, 炎症反应

Abstract: Objective To explore the correlation between serum microRNA 20a and the progress of inflammatory response in children with pneumonia,in order to provide reference for clinical treatment.Methods Totally 90 children diagnosed with pneumonia from March 2015 to February 2017 were selected as pneumonia group (n=90) and 90 healthy children were enrolled in control group (n=90).Routine blood test and erythrocyte sedimentation rate were conducted in children after admission,and specific antibodies against pneumonia were identified by ELISA.Serum concentration of C-reactive protein (CRP) and rheumatoid factor (RF) was quantitatively detected by automatic specific protein analyzer and immunoturbidimetry.Total RNA was separated by TRIzol reagent and reverse transcription cDNA was synthesized from microRNA 20a in serum according to Prime-Script RT kit instructions.Then,the protein extracts were separated by 10%SDS-PAGE gel electrophoresis and transferred to PVDF membrane to express microRNA 20a protein in 20a.Finally,Kolmogorov-Smirnov test was used to analyze the correlation between the expression of microRNA 20a in serum and inflammatory response in children with pneumonia. Results Compared with the control group,serum levels of IgG and IgA in children with pneumonia increased,but IgM did not change significantly.Specific protein analysis showed that serum CRP,RF and IL-17 levels and the expression level of microRNA 20a in pneumonia group were higher than those in control group (P<0.05).The correlation analysis between microRNA 20a parameters and IL-17 level showed positive correlation and a steep correlation curve (r=0.571,P＝0.002). Conclusions Serum expression level of microRNA 20a may be related to the pathogenesis of pneumonia inflammation.MicroRNA 20a is promising to be a marker for the diagnosis of pediatric pneumonia and can be used as an indicator for the poor prognosis of pediatric pneumonia patients.

Key words: microRNA 20a, pediatric pneumonia, inflammatory reactions