中国儿童保健杂志 ›› 2018, Vol. 26 ›› Issue (2): 158-162.DOI: 10.11852/zgetbjzz2018-26-02-12

• 基础科研论著 • 上一篇    下一篇

ω-3多不饱和脂肪酸对脂多糖所致新生大鼠脑损伤Toll-NF-κB信号通路调节作用

石计朋1, 栗延伟2, 宋亚辉3, 张利利4, 桑桂梅1, 郭洪旭1, 王卫卫1, 唐成和1, 黄丽密5   

  1. 1 新乡医学院第一附属医院新生儿科,河南 新乡 453100
    2 新乡医学院第三附属医院神经内科,河南 新乡 453000
    3 南阳市第二人民医院超声科,河南 南阳 473000
    4 新乡医学院第一附属医院消化内科,河南 新乡 4531 00
    5 温州医科大学附属第一医院儿科,浙江 温州 325000
  • 收稿日期:2017-12-28 发布日期:2018-02-10 出版日期:2018-02-10
  • 通讯作者: 黄丽密,E-mail:hlm0201262@126.com
  • 作者简介:石计朋(1981-),男,河南人,主治医师,硕士学位,博士研究生在读,主要研究方向为新生儿疾病。
  • 基金资助:
    河南省医学科技攻关计划项目(201602148);河南省神经修复重点实验室开放课题(HNSJXF-2016-017)

Effect of ω-3 PUFAs on regulation of Toll-like receptor 4 and nuclear factor-ΚB signaling pathway in neonatal rats with brain injury induced by LPS

SHI Ji-peng1, LI Yan-wei2, SONG Ya-hui3, ZHANG Li-li, SANG Gui-mei1, GUO Hong-xun1, WANG Wei-wei1, TANG Cheng-he1, Huang Li-mi4   

  1. 1 Department of Neonatology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453100,China
    2 Department of Neurology,The Third Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453000
    3 Department of Echo Lab,Nanyang Second People's Hospital,Nayang,Henan 473000,China
    4 Department of Gastroenterology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453100,China
    5 Department of Pediatrics,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325000,China
  • Received:2017-12-28 Online:2018-02-10 Published:2018-02-10
  • Contact: HUANG Li-mi,E-mail:hlm0201262@126.com

摘要: 目的 探讨ω-3 PUFAs在脂多糖(LPS)诱导的新生大鼠脑损伤中对TLR4、NF-κB信号通路的调节作用。方法 将96只SD新生大鼠按随机数字法分为对照组,ω-3组,ω-6组和LPS组。4组大鼠分别采用生理盐水或LPS腹腔注射后,立即注射生理盐水或不同的脂肪乳剂,使用时间分别为1天或5天,建立感染所致新生大鼠脑损伤模型;1天或5天后处死新生大鼠,检测TLR4 mRNA、NF-κB mRNA和对应蛋白水平的变化。结果 LPS注射后的第1天、第5天,新生大鼠TLR4 mRNA、NF-κB mRNA表达及其对应的蛋白水平均明显高于对照组(P均<0.05)。1 d 时ω-3组TLR4 mRNA、NF-κB mRNA表达及其对应的蛋白水平均低于ω-6组和LPS组(P均<0.05)。5 d时ω-3组TLR4 mRNA、NF-κB mRNA表达及其对应的蛋白水平均低于ω-6组和LPS组(P均<0.05)。不论第1 d和第5 d,ω-3 PUFAs均可明显降低TLR4 mRNA、NF-κB mRNA表达及其对应的蛋白水平(P均<0.05);而ω-6 PUFAs能提高TLR4 mRNA、NF-κB mRNA表达及对应的蛋白水平(P均<0.05)。结论 ω-3 PUFAs 能下调 TLR4、NF-κB活性,在LPS所致的脑损伤中具有抗炎作用。

关键词: 新生大鼠, ω-3PUFAs, ω-6PUFAs, 脑损伤, 脂多糖, TLR4、NF-κB

Abstract: Objective To investigate the effects of ω-3 PUFAs on regulation of Toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) signaling pathway in neonatal rats with brain injury induced by LPS. Methods Totally 96 neonatal Sprague-Dawley rats were divided into control group,ω-3 group,ω-6 group,and LPS group.Intraperitoneal injection with saline or LPS was administered and immediately saline or fat emulsions was injected to establish a model of rat with brain injury induced by infection and the duration time was 1 day or 5 days respectively.Those groups were sacrificed at 1d or 5 d after intraperitoneal injection with saline or fat emulsions.Expression levels of TLR4 mRNA and NF-κB mRNA were examined by real-time PCR,meanwhile the levels of TLR4,and NF-κB in the hippocampus were measured by Western Blot. Results The expressions of TLR mRNA and NF-κB mRNA and the protein level of TLR4 and NF-κB in the hippocampus were significantly different in four groups(all Pˉ0.05).TLR mRNA and NF-κB mRNA and the protein expression levels in ω-3 group were lower than those in ω-6 group and LPS group on 1 d after injection (all Pˉ0.05).And the protein expression levels of TLR mRNA and NF-κB mRNA in ω-3 group were lower than those in ω-6 group and LPS group on five days after injection (all Pˉ0.05).No matter the first day or the fifth day,the expression of TLR mRNA and NF-κB mRNA and the level of TLR4 and NF-κB in ω-3 group were significantly lower than those in ω-6 group(all Pˉ0.05). Conclusion ω-3 PUFAs can down regulate the activity of signaling pathway of TLR4 and NF-κB,so it may has an anti-inflammatory effect on brain injuryinduced by LPS.

Key words: neonatal rats, ω-3 PUFAs, ω-6 PUFAs, brain injury, LPS, TLR4, NF-κB

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