中国儿童保健杂志 ›› 2020, Vol. 28 ›› Issue (6): 672-675.DOI: 10.11852/zgetbjzz2019-0901

• 临床研究与分析 • 上一篇    下一篇

过敏性哮喘患儿外周血单个核细胞mir-29c与Thl/Th2细胞平衡的相关性分析

董天津, 谭华清, 修青永   

  1. 北京市大兴区妇幼保健院儿科,北京 102600
  • 收稿日期:2019-09-21 发布日期:2020-06-10 出版日期:2020-06-10
  • 通讯作者: 谭华清,E-mail:1845680106@qq.com
  • 作者简介:董天津(1978-),女,主治医师,硕士学位,主要从事儿科临床研究工作。
  • 基金资助:
    北京市大兴区妇幼保健院科研项目(2019004)

Correlation between mir-29c and Thl/Th2 cell balance in peripheral blood mononuclear cells of children with allergic asthma

DONG Tian-jin, TAN Hua-qing, XIU Qing-yong   

  1. Department of Pediatrics,Daxing District Maternal and Child Health Hospital,Beijing 102600,China
  • Received:2019-09-21 Online:2020-06-10 Published:2020-06-10
  • Contact: TAN Hua-qing,E-mail:1845680106@qq.com

摘要: 目的 分析过敏性哮喘(AA)患儿外周血单核细胞微小RNA 29c(mir-29c)表达及与CD4+辅助性T细胞1/CD4+辅助性T细胞2(Th1/Th2)平衡的相关性。方法 将2015年10月-2018年12月本院收治的94例AA患儿分为稳定组32例,轻度急性发作组27例,中度急性发作组35例;另取健康体检儿童40例为对照组。采集所有受试者儿童外周血,分离血清和单个核细胞(PBMC),检测mir-29c、Th1/Th2、IFN-γ、IL-4、T盒子转录因子21(T-bet)、GATA结合蛋白3(GATA3)蛋白水平。结果 与对照组比较,稳定组、轻、中度急性发作组AA患儿PBMC中mir-29c水平、Th2亚群比例、GATA3蛋白水平及血清IL-4水平依次升高,PBMC中Th1亚群比例、Th1/Th2、T-bet蛋白水平、T-bet/GATA3及血清IFN-γ水平、IFN-γ/IL-4依次降低,两两比较差异均有统计学意义(P<0.05)。AA患儿外周血mir-29c水平与Th1/Th2、IFN-γ/IL-4、T-bet/GATA3均呈负相关(r=-0.823、-0.789、-0.804,P<0.05)。结论 mir-29c在AA患儿外周血单个核细胞中上调表达,可能通过Th1/Th2平衡影响AA发生。

关键词: 过敏性哮喘, microRNA-29c, CD4+辅助性T细胞1 / CD4+辅助性T细胞2, 外周血, 单个核细胞

Abstract: Objective To analyze the expression of microRNA 29c (mir-29c) in peripheral blood mononuclear cells of children with allergic asthma (AA) and its correlation with the balance of CD4+helper T cell 1/CD4+helper T cell 2 (Th1/Th2).Methods From October 2015 to December 2018,Totally 94 children with AA were divided into stable group (32 cases),mild acute attack group (27 cases) and moderate acute attack group (35 cases),and another 40 healthy children underwent physical examination were selected as the control group.Peripheral blood was collected from all subjects,serum and peripheral blood mononuclear cell (PBMC) were separated,the levels of mir-29c,Th1/Th2,IFN-gamma,IL-4,T-box transcription factor (T-bet) and GATA binding protein 3 (GATA3) were detected.Results Compared with the control group,the levels of mir-29c,Th2 subgroup,GATA3 protein in PBMC and the level of IL-4 in serum of children with AA in stable group,mild and moderate acute attack group increased in turn,while the percentage of Th1 subgroup,Th1/Th2,T-bet protein level,T-bet/GATA3,serum IFN-γ level and IFN-γ/IL-4 in PBMC decreased in turn,and there was significant difference between any two groups (P<0.05).The levels of mir-29c in peripheral blood of children with AA were negatively correlated with Th1/Th2,IFN-γ/IL-4 and T-bet/GATA3 (r=-0.823,-0.789,-0.804,P<0.05).Conclusion Mir-29c is up-regulated in peripheral blood mononuclear cells of children with AA and may affect the occurrence of AA through Th1/Th2 balance.

Key words: allergic asthma, microRNA-29c, CD4+helper T cell 1/CD4+helper T cell 2, peripheral blood, mononuclear cells

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