金属蛋白酶8基因 C-799T多态性位点与自发性早产遗传易感性的病例对照研究

doi:10.11852/zgetbjzz2017-25-02-02

中国儿童保健杂志 ›› 2017, Vol. 25 ›› Issue (2): 112-116.DOI: 10.11852/zgetbjzz2017-25-02-02

金属蛋白酶8基因 C-799T多态性位点与自发性早产遗传易感性的病例对照研究

杨晓¹,彭薇¹,朱丽娜¹,张小爱²,王艳¹

1 中国人民解放军陆军总医院附属八一儿童医院发育生物学实验室,北京 100700;
2 军事医学科学院微生物和流行病学研究所病原微生物生物安全重点实验室,北京 100071

收稿日期:2016-08-12 发布日期:2017-02-10 出版日期:2017-02-10
通讯作者: 王艳,E-mail001wangyan@sina.com
作者简介:杨晓(1981-),女,湖北人,主管技师,博士学位,主要进行儿童遗传性疾病的细胞分子遗传学研究。
基金资助:
国家自然科学基金(81300527)

A case-control study on the MMP-8C-799T and susceptibility to spontaneous preterm birth and premature rupture of membranes

YANG Xiao¹,PENG Wei¹,ZHU Li-na¹,ZHANG Xiao-ai²,WANG Yan¹

1 Developmental Biology Laboratory,Bayi Children's Hospital Affiliated to PLA Army Beijing General Hospital,Beijing 100700,China;
2 State Key Laboratory of Pathogen and Biosecurity Affiliated to the Institute Microbiology and Epidemiology of the Academy of Military Medical Sciences,Beijing,100071,China

Received:2016-08-12 Online:2017-02-10 Published:2017-02-10
Contact: WANG Yan,E-mail:001wangyan@sina.com

摘要/Abstract

摘要： 目的探讨金属蛋白酶8(MMP-8)基因C-799T多态性位点与自发性早产遗传易感性的关联性。方法病例组样本包括753例自发性早产(SPTB)新生儿,对照组包括681例足月新生儿。采用最新的Sequenom MassARRAY SNP检测技术对MMP-8基因C-799T多态性位点进行SNP分型。结果与携带MMP-8基因C-799T 多态性位点CC基因型的个体相比,携带至少一个-799 T等位型(CT+TT基因型)的个体发生SPTB、合并PROM的SPTB(PPROM)以及轻度早产的风险显著降低。与携带至少一个C等位型(CC+CT基因型)的个体相比,TT基因型与不合并PROM的SPTB的患病风险的降低有临界的相关性。结论 MMP-8基因C-799T多态性位点可以影响SPTB的遗传易感性。

关键词: 金属蛋白酶-8, 自发性早产, 胎膜早破, 单核苷酸多态性中图分类号R179 文献标识码A 文章编号1008-6579(2017)02-0112-05 doi10.11852/zgetbjzz2017-25-02-02

Abstract: Objective To investigate the association between the genetic polymorphisms of MMP-8C-799T and susceptibility to spontaneous preterm birth (SPTB). Methods This case-control study enrolled 753 SPTB singleton neonates and 681 term neonates.Polymorphism was genotyped using Sequenom MassARRAY SNP. Result Compared with the CC genotypes,MMP-8-799T-positive genotypes (CT+TT genotypes) were significantly associated with a decreased susceptibility to SPTB and moderate SPTB.The -799T-positive genotypes (CT+TT genotype) were also significantly associated with decreased SPTB susceptibility in preterm neonates with PROM.Comparing with the CC + CT genotypes,-799TT genotype was marginally associated with a decreased SPTB susceptibility in preterm neonates without PROM. Conclusion MMP-8C-799T contributes to the SPTB susceptibility.

Key words: matrix metalloproteinases-8, spontaneous preterm birth, premature rupture of membranes, single nucleotide polymorphisms

中图分类号:

R179

杨晓,彭薇,朱丽娜,张小爱,王艳. 金属蛋白酶8基因 C-799T多态性位点与自发性早产遗传易感性的病例对照研究[J]. 中国儿童保健杂志, 2017, 25(2): 112-116.

YANG Xiao,PENG Wei,ZHU Li-na,ZHANG Xiao-ai,WANG Yan. A case-control study on the MMP-8C-799T and susceptibility to spontaneous preterm birth and premature rupture of membranes[J]. journal1, 2017, 25(2): 112-116.

参考文献

[1] Di RGC,Giardina I,Rosati A,et al.Maternal risk factors for preterm birth:a country-based population analysis[J].Eur J Obstet Gynecol Reprod Biol,2011,159(2):342-346.
[2] Hamilton BE,Martin JA,Ventura SJ.Births:preliminary data for 2012[J].Natl Vital Stat Rep,2013,62(3):1-20.
[3] Romero R,Velez EDR,Kusanovic JP,et al.Identification of fetal and maternal single nucleotide polymorphisms in candidate genes that predispose to spontaneous preterm labor with intact membranes[J].Am J Obstet Gynecol,2010,202(5):431.e1-34.
[4] Moura E,Mattar R,de Souza E,et al.Inflammatory cytokine gene polymorphisms and spontaneous preterm birth[J].J Reprod Immunol,2009,80(1-2):115-121.
[5] Velez DR,Fortunato SJ,Thorsen P,et al.Preterm birth in Caucasians is associated with coagulation and inflammation pathway gene variants[J].PLoS One,2008,3(9):e3283.
[6] Nagase H,Visse R,Murphy G.Structure and function of matrix metalloproteinases and TIMPs[J].Cardiovasc Res,2006,69(3):562-573.
[7] Weiss A,Goldman S,Shalev E.The matrix metalloproteinases (MMPS) in the decidua and fetal membranes[J].Front Biosci,2007,12:649-659.
[8] Nien JK,Yoon BH,Espinoza J,et al.A rapid MMP-8 bedside test for the detection of intra-amniotic inflammation identifies patients at risk for imminent preterm delivery[J].Am J Obstet Gynecol,2006,195(4):1025-1030.
[9] Wang Y,Yang X,Zheng Y,et al.The SEPS1 G-105A polymorphism is associated with risk of spontaneous preterm birth in a Chinese population[J].PLoS One,2013,8(6):e65657.
[10] Wang Y,Yang X,et al,A MCP-1 promoter polymorphism at G-2518A is associated with spontaneous preterm birth[J].Mol Genet Genomics,2015,290(1):289-296.
[11] 时春燕,漆洪波,杨慧霞.胎膜早破的诊断与处理指南(2015)[J].中华妇产科杂志,2015,3(1):3-8.
[12] Biggio JRJ,Ramsey PS,Cliver SP,et al.Midtrimester amniotic fluid matrix metalloproteinase-8 (MMP-8) levels above the 90th percentile are a marker for subsequent preterm premature rupture of membranes[J].Am J Obstet Gynecol,2005,192(1):109-113.
[13] Chaiworapongsa T,Hong JS,Hull WM,et al.Amniotic fluid concentration of surfactant proteins in intra-amniotic infection[J].J Matern Fetal Neonatal Med,2008,21(9):663-670.
[14] Park CW,Kim SM,Park JS,et al.Fetal,amniotic and maternal inflammatory responses in early stage of ascending intrauterine infection,inflammation restricted to chorio-decidua,in preterm gestation[J].J Matern Fetal Neonatal Med,2014,27(1):98-105.
[15] Shim SS,Romero R,Hong JS,et al.Clinical significance of intra-amniotic inflammation in patients with preterm premature rupture of membranes[J].Am J Obstet Gynecol,2004,191(4):1339-1345.
[16] Maymon E,Romero R,Chaiworapongsa T,et al.Amniotic fluid matrix metalloproteinase-8 in preterm labor with intact membranes[J].Am J Obstet Gynecol,2001,185(5):1149-1155.
[17] Wang H,Parry S,Macones G,et al.Functionally significant SNP MMP8 promoter haplotypes and preterm premature rupture of membranes (PPROM)[J].Hum Mol Genet,2004,13(21):2659-2669.
[18] Fortunato SJ,Menon R,Lombardi SJ.Amniochorion gelatinase-gelatinase inhibitor imbalance in vitro:a possible infectious pathway to rupture[J].Obstet Gynecol,2000,95(2):240-244.
[19] Beeghly-Fadiel A,Zheng W,Lu W,et al.Replication study for reported SNP associations with breast cancer survival[J].J Cancer Res Clin Oncol,2012,138(6):1019-2426.

金属蛋白酶8基因 C-799T多态性位点与自发性早产遗传易感性的病例对照研究

A case-control study on the MMP-8C-799T and susceptibility to spontaneous preterm birth and premature rupture of membranes

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

[1]	王华, 程玉梅, 陈永萍, 侯东敏. 某院近五年婴儿出生发生早产状况及预后分析[J]. 中国儿童保健杂志, 2019, 27(10): 1128-1132.
[2]	董仁静, 金贞爱, 刘梦南, 张晓佳, 金英子. 未足月胎膜早破新生儿脐血中IL-6、TNF-α水平与绒毛膜炎的关系[J]. 中国儿童保健杂志, 2018, 26(2): 206-207.
[3]	刘敬. 胎膜早破对新生儿多器官系统的损害与临床管理[J]. 中国儿童保健杂志, 2017, 25(11): 1081-1085.
[4]	刘梦南,张晓佳综述,金贞爱审校. Pro-ADM、sTREM-1与未足月胎膜早破新生儿感染[J]. 中国儿童保健杂志, 2017, 25(11): 1128-1130.
[5]	谷惠芳,戎小平,刘雪,张焕改,成春苹,刘香,郭玮. Toll样受体4及肿瘤坏死因子α在早产儿外周血水平及其与早产儿脑损伤预后的关系[J]. 中国儿童保健杂志, 2015, 23(11): 1131-1133.