关键词: HSP70/CD80DNA疫苗, CpG ODN, 气道炎症, 哮喘小鼠

Abstract: Objective To study the effect of non-methylated CpG oligodeoxynucleotide (cytosine-phosphate-guanosine-oligodeoxynucleotides, CpG ODN) combined with heat shock protein 70 (heat shock protein 70, HSP70)/CD80DNA vaccine on pulmonary inflammation, serum levels of IL-4, IL-13, IFN-γ and the changes of IL-4, IL-25 and IL-33 in bronchoalveolar lavage fluid(BALF) of asthmatic mice, so as to provide experimental basis for the treatment of asthma by CpG ODN-HSP70/CD80DNA vaccine. Methods Ovalbumin (OVA) and aluminum hydroxide [Al (OH)₃] adjuvant were used to sensitize mice with asthma model.From June to September 2019,totally 32 BALB/c mice aged 6—8 weeks were selected and were randomly divided into four groups, including control group, asthma group, HSP70/CD80DNA vaccine group and combined vaccine group (CpG ODN combined with HSP70/CD80DNA vaccine group).HE staining was used to observe the pathological and morphological changes of lung tissue.The changes of serum levels of IL-4, IL-13, IFN-γ in and IL-4, IL-25, IL-33 in BALF were detected by ELISA. Results HE staining of lung tissue showed that the inflammatory response of lung tissue in combined vaccine group was significantly less than that in the asthma group, and the infiltration of inflammatory cells around the airway was significantly reduced.ELISA results showed that compared with the control group, serum levels of IL-4 and IL-13 in asthma group were significantly higher (P<0.05), and serum IFN-γ level was significantly lower (P<0.05).Compared with the asthma group, serum levels of IL-4 and IL-13 in the Hsp70/CD80DNA vaccine group and the combined vaccine group were significantly lower (P<0.05), and the serum IFN-γ level was significantly higher (P<0.05).Compared with the Hsp70/CD80DNA vaccine group, the serum levels of IL-4 and IL-13 in combined vaccine group were significantly decreased (P<0.05), and the serum IFN-γ were significantly increased (P<0.05).The results of BALF showed that compared with the control group, the levels of BALF IL-4, IL-25 and IL-33 in the asthma group were significantly increased (P<0.05).Compared with the asthma group, the levels of IL-4, IL-25 and IL-33 in BALF in the Hsp70/CD80 DNA vaccine group and the combined vaccine group were significantly decreased (P<0.05).And the levels of IL-33 in BALF in the combined vaccine group were significantly lower than those in the Hsp70/CD80 DNA vaccine group (P<0.05). Conclusion CpG ODN combined with Hsp70/CD80DNA vaccine can inhibit the production of IL-4, IL-13, IL-25 and IL-33 in mice, enhance the production of IFN-γ in mice, and reduce airway inflammation in asthmatic mice.

Key words: HSP70/CD80DNA vaccine, CpG ODN, airway inflammation, asthmatic mice