关键词: 儿童哮喘, 维生素D, T细胞亚群, 细胞因子

Abstract: Objective To analyze the changes and correlation of serum 25-hydroxyvitamin D₃ [25-(OH)D₃], the number of T lymphocytes and related cytokines in children with asthma at different stages, and to explore the possible mechanism of 25-(OH)D₃ involved in the pathogenesis and progression of asthma. Methods From January 2018 to December 2019, a total of 105 children with asthma and 30 healthy children in Sichuan Provincial People's Hospital were selected into this study, including 25 cases of acute attack, 40 cases of chronic duration and 40 cases of remission.The level of 25-(OH)D₃ were detected by electrochemical stripping voltammetry and the levels of T lymphocyte subsets and their cytokines IFN- γ, IL-4 and IL-10 were detected by flow cytometry. Results In children with asthma, serum 25-(OH)D₃ level decreased significantly, the number of CD3, CD4/CD8 cells as well as the secretion level of IFN-γ and IL-10 decreased, while the level of IL-4 secretion increased.Changes were most pronounced during acute episodes, followed by chronic persistence, and closest to normal during remission.The level of serum 25-(OH) D₃ in children with asthma at different stages was positively correlated with the level of IFN-γ and IL-10, and negatively correlated with the level of IL-4. Conclusions Vitamin D, as an immunomodulatory hormone, is closely related to the onset and disease control of asthma.Monitoring serum 25-(OH) D₃ level of asthmatic patients is helpful for the judgment and the treatment of the disease condition, providing a new monitoring method for the clinical practice.

Key words: childhood asthma, vitamin D, T cell subsets, cytokines