• 基础科研论著 •

### 干细胞来源外泌体对早产仔鼠运动影响的研究

1. 1.西安交通大学第一附属医院妇产科,陕西 西安 710061;
2.安康市妇幼保健院妇产科,陕西 安康 725000
• 发布日期:2022-04-29
• 通讯作者: 宗璐,E-mail:catherazong@163.com
• 作者简介:王娟(1994-),女,陕西人,住院医师,本科学历,主要研究方向为妊娠合并感染性疾病。
• 基金资助:
国家自然科学基金项目(81701532;81601316);陕西省卫生健康科研基金项目(2018D055);步长-西安交通大学第一附属医院联合基金项目(BC2017-08);本香基金项目资助

### Effects of exosomes derived from stem cells on the movement of premature rats

WANG Juan*, ZONG Lu

1. *Obstetrics and Gynecology of First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shaanxi 710061,China; Obstetrics and Gynecology of Ankang Maternal and Child Health Hospital, Ankang, Shaanxi 725000, China
• Published:2022-04-29
• Contact: ZONG Lu, E-mail:catherazong@163.com

Abstract: Objective To discuss the effect of exosomes derived from human umbilical cord blood mesenchymal stem cells (Hu-MSCs) on movement ability of the intrauterine infection offspring rats and its mechanism, so as to provide new ideas for treating intrauterine infection and motor function impariment. Methods Ninety pregnant rats were randomly divided into three groups [the experimental group, the lipopolysaccharide(LPS) group and the control group].On the 15th day of embryo(E15), dams of the experimental group were given 0.5 ml 1×106 ng/μl intraperitoneal injection of exosomes, dams of the LPS group and the control group were given intraperitoneal injection of the same amount of physiological saline solution. On the 17th day of embryo(E17), dams of the experimental group and the LPS group were given intrauterine injection of 100 μl 25 μg/μl lipopolysaccharide (LPS) solution, and dams of the control group were given intrauterine injection of the same amount of normal saline. On the 18th day of embryo(E18), fifteen pregnant rats were randomly selected from the three groups and killed. Then ELISA method was used to check the change in the IL-4, IL-10 and IL-12 of the amniotic fluid of the pregnant rats and serum of the offspring. The remaining 15 pregnant rats in each group gave birth naturally and their pregnancy outcomes were observed. And 10 offspring were selected randomly to evaluate their movement ability by using Suspension Test and Basso-Beatie-Bresnahan Scale (BBB Scale) once 15 days old and once 30 days old. Results 1) The levels of IL-4 and IL-10 in the amniotic fluid of the dams and serum of the offspring in the experimental group were significantly elevated from those in the LPS group (t=8.26, 3.97, 7.66, 4.81, P<0.05). The levels of IL-12 in the amniotic fluid of the dams and serum of the offspring in the experimental group were significantly reduced from those of the LPS group (t=3.14, 6.68, P<0.05). 2) In suspension test, the grasp time of the 15-day-old and 30-day-old offspring in the LPS group was significantly shorter than those of the control group(t=8.166,16.283, P<0.05), which were also significantly shorter than those of the experimental group (t=4.867, 6.994, P<0.05).The BBB score of the 15-day-old and 30-day-old offspring in the LPS group were significantly lower than those of the control group (t＝7.394, 16.283, P<0.05),which were also significantly lower than those of the experimental group (t=4.096, 7.004, P<0.05). Conclusion The exosomes derived from human umbilical cord blood mesenchymal stem cells (Hu-MSCs) may improve the impairment of premature offspring pup's motor function by reducing the inflammatory response in pregnant and offspring rats.