Abstract: Objective To detect the dynamic influence of nervous system development in neonatal rats by the intrauterine human cytomegalovirus (HCMV) infection. Methods The SD rat model of intrauterine HCMV infection was established, then the rats selected from experimental group and the control group randomly were done neuroethology test, such as Morris water maze test, hanging test, slope test, the open field test etc at the age of 1, 3 and 6 months respectively.Before and after the experiment, the light microscope and electron microscopy were used to observe their dynamic pathological change of brain tissue separately. Results Compared with normal control group, the Morris water maze escape latency, swimming distance of the experimental group at the age of 1, 3, 6 months were significantly higher than those of the control group, at the same time cross the platform number was significantly lower than control group, and with the increase of age, the cross platform ability was drop obviously (F=45.09, P<0.01);Motor functionrats' hanging test scores were (1.49±0.53), (1.23±0.46), (0.93±0.35) in infected group at the age of 1, 3 and 6 months, while those in the control group were (2.29±0.49), (2.14±0.69), (1.98±0.25), there were significant differences between two groups(samples nonparametric test, P all<0.001), suggested sport function of infected neonatal rat was decreased significantly.Open filed resultoffspring's across the grid number were (44.0±7.32), (37.40±9.56), (26.62±8.87) in infected group in 1, 3 and 6 months of age, while those in the control group were (57.07±9.65), (55.27±9.36), (54.27±8.14), there were significant differences between two groups (P all <0.05).Pathological results showed that along with the age growth, the hippocampus structure and nerve myelin of the experimental group offspring were aggravate gradually. Conclusion Intrauterine HCMV infection may result in the abnormal neural behavior of neonatal rat, and the impairment of learning and memory, motor function and emotional behavior development is aggravate gradually.

Key words: human cytomegalovirus, intrauterine infection, neonatal rats, neurobehavior

摘要： 目的 探讨宫内人巨细胞病毒(human cytomegalovirus, HCMV)感染对新生大鼠神经系统发育的动态影响。方法 建立宫内HCMV感染SD大鼠模型, 于1、3、6月龄时分别对实验组与对照组进行Morris 水迷宫、悬吊实验、斜坡实验、旷场实验等神经行为学检测, 实验前后分别采用光镜、电镜观察两组脑组织病理动态变化特点。结果 与正常对照组比较, 实验组1、3、6月龄时的Morris 水迷宫逃避潜伏期、游泳距离均显著高于对照组, 同时其穿越平台次数显著低于对照组, 且随着年龄增加, 其穿越平台能力下降越明显(F=45.09, P<0.01);运动能力方面实验组大鼠1、3、6月龄, 悬吊实验得分(1.49±0.53、1.23±0.46、0.93±0.35)显著低于对照组(1.98±0.25、2.14±0.69、2.29±0.49), 差异均具有统计学意义(P均<0.001, 两独立样本非参数检验);表明宫内HCMV感染新生大鼠其运动能力显著降低;旷场试验结果实验组大鼠1、3、6月龄, 跨格子数(44.0±7.32、37.40±9.56、26.62±8.87)显著低于对照组(54.27±8.14、55.27±9.36、57.07±9.65), 差异具有统计学意义(P均<0.05)。病理结果显示随年龄增长, 实验组子代鼠的海马结构及神经髓鞘呈进行性破坏。结论 宫内HCMV感染可以导致新生大鼠出现神经行为异常, 表现为学习记忆、运动功能和情绪行为发育的异常加重。

关键词: 人巨细胞病毒, 宫内感染, 新生大鼠, 神经行为