Polymorphism of vitamin D receptor gene Fok I in the Korean children in Yanbian district

doi:10.11852/zgetbjzz2015-23-04-05

Abstract

Abstract: Objective To explore the polymorphism distribution of vitamin D receptor(VDR)gene Fok Ⅰin the Korean children in Yanbian Province for further study on relation between VDR gene polymorphisms and metabolic bone disease. Methods A total of 98 Korean children were recruited and collected oral epithelial cells.Genome DNA for fluorescence quantitative PCR to detect SNP of Fok Ⅰ were extracted.The results were compared with the distribution frequency of Fok Ⅰin other nations or races. Result The frequency of Fok Ⅰ( ff,Ff,FF ) in Korean children were 23%,40%,37% respectively,which were significant difference to Achangs,Dongxiang nationality,Americans and Nigerian (P<0.05). Conclusion Fok Ⅰ polymorphism distribution in Korean children exhibits its own characteristics.

Key words: vitamin D receptor, polymorphism, Korean

摘要： 目的了解延边地区朝鲜族儿童维生素D受体(vitamin D receptor,VDR)基因Fok I位点的多态性分布特点,为进一步研究VDR基因多态性与骨代谢疾病的关系打下基础。方法应用荧光定量PCR方法,检测98名延边地区朝鲜族儿童的VDR基因Fok I位点多态性,分析该基因型和等位基因频率分布,并与已报道的其他民族、种族的数据进行比较。结果本研究人群VDR基因ff、Ff、FF基因型的分布频率分别为23%、40%、37%,该分布情况与国内云南阿昌族、甘肃东乡族以及国外美国黑人、尼日利亚人的差异具有统计学意义(P<0.05)。结论延边地区朝鲜族儿童VDR基因Fok I位点基因型和等位基因的频率分布特征有其自身的特点。

关键词: 维生素D受体, 基因多态性, 朝鲜族

CLC Number:

R725

QUAN Jing-hua,LU Yan-qiang,LI Ying,JIN Jing-ji,SONG Jin-xia. Polymorphism of vitamin D receptor gene Fok I in the Korean children in Yanbian district[J]. journal1, 2015, 23(4): 351-353.

全京花,鲁衍强,李瑛,金京姬,宋锦霞. 延边地区朝鲜族儿童维生素D受体基因Fok I位点多态性研究[J]. 中国儿童保健杂志, 2015, 23(4): 351-353.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/10.11852/zgetbjzz2015-23-04-05

https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/Y2015/V23/I4/351

References

[1] Yallg CY,Leung PS,Adamopoulos IE,et al.The implication of vitamin D and autoimmunity:a comprehensive review[J].Clin Rev Allergy Immunol,2013,45(2):217-226.
[2] SmoldeIs J,Peelen E,Thewissen M,et al.The relevance of vitamin D receptor gene polymorphisms for vitamin D research in multiple sclerosis[J].Autoimmun Rev,2009,8(7):621-626.
[3] Eelen G,Gysemans C,Verlinden L,et al.Mechanism and potential of the growth-inhibitory actions of vitamin D and analogs[J].Curr Med Chem,2007,14(17):1893-1910.
[4] Kostner K,Denzer N,Muller CS,et al.The relevance of vitamin D receptor(VDR)gene polymorphisms for cancer:a review of the literature[J].Anticancer Res,2009,29:3511-3536.
[5] 李继梅,卢华君,李海林,等.阿昌族与汉族维生素D受体基因FokI多态性[J].中华医学遗传学杂志,2003,20(4):315-317.
[6] 邢少姬,周立社,许秀举.蒙古族人群维生素D受体基因FokI多态性[J].中华医学遗传学杂志,2006,23(2):236-237.
[7] 郭敏,李宇宁,凌继祖,等.维生素D受体基因BsmI及FokI位点单核苷酸多态性与维生素D缺乏性佝偻病的相关性[J].临床儿科杂志,2012,30(11):1047-1050.
[8] Arai H,Miyamoto K,Taketani Y,et al.A vitamin D receptor gene polymorphism in the translation initiation codon:effect on protein activity and relation to bone mineral desity in Japanese women[J].J Bone Miner Res,1997,12:915-921.
[9] Harris SS,Eccleshall TR,Gross C,et al.The vitamin D receptor start codon polymorphism (Fok I) and bone mineral density in premenopausal American black and white women[J].J Bone Miner Res,1997,12:1043-1048.
[10] Eccleshall TR,Garnero P,Gross C,et al.Lack of correlation between start codon polymorphism of the vitamin D receptor gene and bone mineral density in premenopausal French women:the OFFLY study[J].J Bone Miner Res,1998,13:31-35.
[11] Fischer PR,Thacher TD,Pettifor JM.Vitamin D receptor polymorphisms and nutritional rickets in Nigerian children[J].J Bone Miner Res,2000,15:2206-2210.
[12] Eelen G,Gysemans C,Verlinden L,et al.Mechanism and potential of the growth-inhibitory actions of vitamin D and analogs[J].Curr Med Chem,2007,14(17):1893-1910.
[13] Adrianna M,Margarita L,Mariusz W,et al.Vitamin D receptor gene BsmI,FokI,ApaI and TaqI polymorphisms and the risk of systemic lupus erythematosus[J].Mol Biol Rep,2013,40(2):803-810.
[14] Sharif BM,Frank CG,Cedric FG,et al.Is there a role of vitamin D deficiency in type 1 diabetes of children?[J].Am J Prev Med,2010,39(2):189-190.
[15] Kostner K,Denzer N,Muller CS,et al.The relevance of vitamin D receptor(VDR)gene polymorphisms for cancer:a review of the literature[J].Anticancer Res,2009,29:3511-3536.