Abstract: Objective To analyze the expression level of microRNA-155(miRNA-155) in asthmatic predictive index (API) positive and negative children with recurrent wheezing, and to explore the relationship between miRNA-155 and wheezing as well as eosinophil count. Methods A total of 40 children with recurrent wheezing in Yuhuan People′s Hospital from January 2019 to October 2020 were enrolled in the wheezing group, including 27 API positive children and 13 API negative children. Meanwhile, 40 healthy children were selected into the control group. The levels of miRNA-155, eosinophils, IFN-γ and IL-4 in peripheral blood of wheezing group and control group were tested. Results The levels of miRNA-155 and IFN-γ/IL-4 in wheezing group were significantly lower than those in control group(t=2.307, 3.839, P<0.05), while the number of eosinophils in wheezing group was significantly higher than that in the control group(t =5.278, P＜0.05). The miRNA-155 level and the IFN-γ/IL-4 value of API positive children in the wheezing group were significantly lower than those of API negative and control children (H=13.580, F=9.731,P<0.05). In the wheezing group, the number of eosinophils of API positivechildren was significantly higher than that of API negative and control children(F=22.303, P＜0.05). In the wheezing group, the level of miRNA-155 in API positive children was negatively correlated with the number of wheezing(r＝-0.566, P<0.05).The level of miRNA-155 was negatively related to eosinophil count in the wheezing group(r＝-0.630, P<0.05). Conclusion The decrease of miRNA-155 level may be a risk factor for wheezing, and the role of miRNA-155 in wheezing development may be related to eosinophils.

Key words: microRNA-155, wheezing, asthma predictive index, children, asthma

摘要： 目的 分析miRNA-155在哮喘预测指数(API)阳性和阴性反复喘息儿童中的表达水平,探讨微小RNA-155(miRNA-155)与喘息及嗜酸性粒细胞计数的关系。方法 选取2019年1月—2020年10月在玉环市人民医院住院治疗的反复喘息儿童40例为喘息组,其中API阳性儿童27例,API阴性儿童13例;同时选择健康体检儿童40例为对照组。检测喘息组和对照组儿童外周血miRNA-155、嗜酸性粒细胞、IFN-γ和IL-4水平。结果 喘息组miRNA-155水平、IFN-γ/IL-4值显著低于对照组 (t＝2.307,3.839,P＜0.05),喘息组嗜酸性粒细胞数明显高于对照组(t =5.278,P＜0.05);喘息组中API阳性儿童miRNA-155水平、IFN-γ/IL-4值显著低于API阴性及对照组儿童(H =13.580,F=9.731,P＜0.05),喘息组中API阳性儿童嗜酸性粒细胞明显高于API阴性及对照组儿童(F=22.303,P＜0.05);喘息组中API阳性儿童miRNA-155水平与喘息次数呈负相关(r＝-0.566,P＜0.05),喘息组miRNA-155水平与嗜酸性粒细胞计数呈负相关(r＝-0.630,P＜0.05)。结论 miRNA-155水平下降可能是喘息发生的危险因素,且miRNA-155在喘息发生发展中的作用可能与嗜酸性粒细胞有关。

关键词: 微小RNA-155, 喘息, 哮喘预测指数, 儿童, 哮喘