%0 Journal Article %A HUANG Jun-rong %A LI Xiu-hong %A TI Yun-xing %A ZHOU Wen-jia %A LIANG Sui-xin %T Protective effect and mechanism of delayed mild hypothermia on white matter injury inoxygen-glucose deprivation and restoration newborn rats %D 2022 %R 10.11852/zgetbjzz2022-0036 %J Chinese Journal of Child Health Care %P 1083-1087 %V 30 %N 10 %X Objective To analyze the protective effect and possible mechanism of delayed mild hypothermia on white matter injury in oxygen-glucose deprivation and restoration newborn rats. Methods The models of whole brain perfusion and oxygen glucose deprivation were established in 40 neonatal rat pups. Forty neonatal rats were randomly divided into four groups and treated with mild hypothermia (32 ℃) for 0 h, 24 h, 48 h, 72 h, with 10 rats in each group. The expression of myelin basic protein (MBP) was detected by Western Blot.The expression of microglia was identified by immunofluorescence.The concentrations of TNF-α and IL-6 were determined by ELISA. Results The level of MBP protein expression at 32 ℃ for 24 h, 48 h and 72 h was significantly higher than that at 0 h in the control group (F=478.007, t=-18.180、22.940,-29.670,P<0.001), and there was a significant positive correlation between the protein expression level and the duration of delayed mild hypothermia.With the extension of delayed mild hypothermia time, the activation degree of microglia was inhibited. The number of Iba-1 positive cells at 24 h, 48 h and 72 h was significantly lower than that at 0 h in control group (F=99.892, t=5.879, 9.295, 18.760,P<0.001), showing that increased fluorescence expression level was negatively correlated with delayed mild hypothermia time. The concentrations of TNF-α and IL-6 were significantly lower than those of the control group at 0 h (TNF-α:F=454.197, t=2.184, 15.300, 15.000, P<0.05;IL-6:F=123.995, t=5.650,13.120, 13.910, P<0.001), indicating that the concentrations of IL-6 was negatively correlated with the time of delayed hypothermia. Conclusion Delayed mild hypothermia can protect the white matter injury of oxygen-glucose deprivation and reoxygen-glucose newborn mice, and its mechanism may be related to inhibiting microglial cell activation and down-regulating inflammatory response. %U https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/10.11852/zgetbjzz2022-0036