Expression and clinical value of miR-138 and miR-338 in peripheral blood of children with spastic cerebral palsy
- LIU Hong-xing, NIU Guo-hui, CUI Bo, ZHANG Guang-yu, WANG Xin
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Objective To detect the expression levels of microRNA-138 (miR-138) and microRNA-338 (miR-338) in children with spastic cerebral palsy and explore the clinical value. Methods A total of 64 children with spastic cerebral palsy who were treated in the Third Affiliated Hospital of Zhengzhou University from March 2018 to April 2020 were selected into spastic cerebral palsy group. Meanwhile,62 healthy children were selected as healthy control group. The expression levels of miR-138 and miR-338 were detected by real-time quantitative PCR (qRT-PCR),the serum levels of tumor necrosis factor-α (TNF-α),C-reactive protein (CRP),nerve growth factor (NGF) and endothelin (ET) were detected by enzyme-linked immunosorbent assay (ELISA). Pearson method was used to analyze the correlation between serum miR-138,miR-338 and TNF-α,CRP,NGF,ET expression levels in children with spastic cerebral palsy Receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of serum miR-138 and miR-338 in spastic cerebral palsy. Multivariate Logistic regression analysis was used to analyze the influencing factors of spastic cerebral palsy. Results The expression levels of miR-138,miR-338,TNF-α,CRP and ET in serum in spastic cerebral palsy group were higher than those in healthy control group,while NGF expression level was lower than that in control group(t=9.598,9.592,11.039,42.397,14.172,5.744,P＜0.05). There was a positive correlation between serum miR-138 and miR-338 in children with spastic cerebral palsy (r = 0.687,P < 0.05),and they were positively correlated with TNF-α,CRP,ET levels (miR-138:r=0.519,0.483,0.494;miR-338:r=0.506,0.492,0.516,P＜0.05),and negatively correlated with NGF level (r=-0.522,-0.496,P＜0.05). The area under the curve (AUC) of serum miR-138 and miR-338 in the diagnosis of spastic cerebral palsy was 0.882 and 0.858,the cut-off value was 1.189 and 1.406,the specificity was 75.8%,93.5%,and the sensitivity was 87.5% and 67.2%,respectively.The AUC of the combined diagnosis was 0.950,the specificity was 90.3%,and the sensitivity was 93.8%. Logistic regression analysis that miR-138,miR-338 and NGF were protective factors of spastic cerebral palsy [OR (95%CI):2.280 (1.742-2.983),2.222 (1.637-3.016),P<0.05],and NGF was risk factor of spastic cerebral palsy [OR (95%CI):0.720 (0.527-0.984),P＜0.05]. Conclusions The serum expression levels of miR-138 and miR-338 in children with spastic cerebral palsy are significantly up-regulated,and they may play an important role in the occurrence and development of spastic cerebral palsy.