Research on the correlation among vitamin D,bone morphogenetic protein-2 and osteoblast function in growth hormone deficiency children
- YI Xiao-qing, HAN Tian-tian, LIU Hong-hong, WANG Si-si, ZHANG Rong, WANG Ai-rong, XIAO Yan-feng
Related Articles |
Objective To clarify the correlation among vitamin D,bone morphogenetic protein-2(BMP-2) and osteoblast function in children with growth hormone deficiency(GHD),in order to provide theoretical reference for the complementary treatment of short stature in children. Methods A total of 83 GHD children and 40 normal children admitted in the Pediatrics Department of the Second Affiliated Hospital of Xi'an Jiaotong University from March 2012 to March 2013 were enrolled in this study. According to the Tanner staging and age,GHD children were divided into 2 sub-groups,prepuberty group and puberty group. Their body height,body height standard deviation (HtSDS),bone age were measured. Serum levels of 25(OH)D3,BMP-2,osteocalcin (OC) and procollagen Ⅰ N-terminal propeptide (PINP) were tested by enzyme linked immunosorbent assay (ELISA) in GHD children,and the results were compared with healthy control. The relationship among 25(OH)D3,BMP-2,OC,PINP,GH,HtSDS,the difference between bone age and age were analyzed by Pearson correlation analysis. Results The prepubertal GHD and the pubertal GHD children had significant lower levels of 25(OH)D3,BMP-2,OC and PINP than the controls(t=4.282,7.162,3.940,4.832,P＜0.05). It was significant in the correlation between 25(OH)D3 and bone formation markers (OC,PINP,BMP-2 and GH) in GHD children (r=0.481,0.473,0.324,0.545, P＜0.05),as well as the correlation between BMP-2and bone formation markers (OC,PINP and GH) in GHD children(r=0.322,0.415,0.619, P＜0.05).But the correlation among 25(OH)D3,BMP-2 and HtSDS and the difference betweenbone age and age were not significant in GHD children(P＞0.05). Conclusions Bone formation in GHD children is lower than healthy controls. The low levels of osteogenic markers in GHD children,like OC and PINP,are related to its low vitamin D and BMP-2 levels. The low level of GH has an impact on the levels of vitamin D and BMP-2.