RHuEPO对大鼠皮肤急性创面愈合过程中血管生成素-1变化的影响

中国临床药理学与治疗学

RHuEPO对大鼠皮肤急性创面愈合过程中血管生成素-1变化的影响

徐姝娟，吕大伦，陈雷，丁伟，王合丽，张炜，王帅

皖南医学院弋矶山医院，芜湖 241001，安徽

出版日期:2017-04-26 发布日期:2017-04-26
通讯作者: 吕大伦，男，主任医师，副教授，硕士生导师，研究方向：创面修复。 E-mail：ldl0776@126.com
作者简介:徐姝娟，女，本科，副主任护师，研究方向：慢性创面修复与护理。
基金资助:
安徽省高校省级自然科学研究项目（KJ2013Z335）；安徽省芜湖市科技计划项目（2012hm30）

Effect of rHuEPO on the change of Ang-1 during acute skin wound healing in rats

XU Shujuan, LV Dalun, CHEN Lei, DING Wei, WANG Heli, ZHANG Wei, WANG Shuai

Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China

Online:2017-04-26 Published:2017-04-26

摘要/Abstract

摘要：

目的: 探讨重组人促红细胞生成素(rHuEPO)对大鼠皮肤切创形成的急性创面愈合过程中血管生成素-1（Ang-1）表达的影响。方法: 健康SD雄性72只大鼠建立急性创面动物模型，随机分成低剂量组(50 U/mL rHuEPO)，中剂量组(100 U/mL rHuEPO)，高剂量组(150 U/mL rHuEPO)和生理盐水(NS)对照组，造模同时进行外敷不同剂量rHuEPO及生理盐水治疗。观察每组大鼠创面愈合情况，并于伤后3、7、14 d取创面皮肤标本，采用免疫组织化学方法染色检测创面CD31、Ang-1的表达。结果: 免疫组化结果为CD31、Ang-1在高剂量和中剂量药物治疗组皮肤组织中高表达，在同一时相点与NS对照组、低剂量组有统计学差异（P<0.05）。结论: rHuEPO能促进大鼠皮肤急性创面愈合过程中Ang-1表达，并促进创面微血管形成,从而加速急性创面愈合。

关键词: 重组人促红细胞生成素, CD31, 血管生成素-1, 急性创面愈合

Abstract:

AIM:To investigate the effects of recombinant human erythropoietin (rHuEPO) on Ang-1 during acute skin wound healing in rats. METHODS: 72 healthy male SD rats were selected to establish animal model of acute wounds, and were randomly divided into low dose group(50 U/mL rHuEPO), middle dose group(100 U/mL rHuEPO), high dose group (150 U/mL rHuEPO) and normal saline group (NS).Different doses of rHuEPO(external application) and saline were administered at the same time of modeling. Healing of the wound was observed in rats in each group. Skin samples 3 d,7 d,14 d after injury were collected to detect the changes of CD31 and Ang-1under immunohistochemistry staining. RESULTS: Compared with control group and low dose rHuEPO group,results of immunohistochemistry indicated higher expression of CD31and Ang-1 in high dose and middle dose rHuEPO group after 3 d and 7 d treatment (P<0.05). CONCLUSION: rHuEPO can increase the expression of Ang-1 and the angiogenesis of rats during healing, thereby accelerating wound healing process.

Key words: recombinant human erythropoietin, CD31, Ang-1, acute wound healing

中图分类号:

R965.2

徐姝娟，吕大伦，陈雷，丁伟，王合丽，张炜，王帅. RHuEPO对大鼠皮肤急性创面愈合过程中血管生成素-1变化的影响[J]. 中国临床药理学与治疗学.

XU Shujuan, LV Dalun, CHEN Lei, DING Wei, WANG Heli, ZHANG Wei, WANG Shuai. Effect of rHuEPO on the change of Ang-1 during acute skin wound healing in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics.