欧米伽3脂肪酸对硫代乙酰胺诱导的大鼠肝纤维化的保护作用

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (8): 892-898.

欧米伽3脂肪酸对硫代乙酰胺诱导的大鼠肝纤维化的保护作用

曾熹，胡杨柳子，吴亚敏，陈志恒

中南大学湘雅三医院健康管理科，长沙 410013，湖南

收稿日期:2016-11-22 修回日期:2017-04-25 出版日期:2017-08-26 发布日期:2017-08-18
通讯作者: 陈志恒，女，教授，硕士生导师，研究方向：慢性病分级管理、胸苷激酶1（TK1）肿瘤早期风险研究等。 E-mail：873127193@qq.com
作者简介:曾熹，男，硕士，医师，研究方向：常见外科疾病尤其肝胆外科疾病的诊治。 E-mail：Zengxi-cs@163.com

Protective effect of omega-3 fatty acids on hepatic fibrosis of rats induced by thioacetamide

ZENG Xi, HUYANG Liuzi, WU Yamin, CHEN Zhiheng

The Health Management Center of the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, China

Received:2016-11-22 Revised:2017-04-25 Online:2017-08-26 Published:2017-08-18

摘要/Abstract

摘要：

目的: 探讨鱼油中欧米伽3脂肪酸对硫代乙酰胺 (TAA)诱导大鼠肝纤维化的保护作用。方法: 60只大鼠随机分为4组。对照组接受生理盐水处理，TAA组接受250 mg/kg剂量的TAA处理，欧米伽3脂肪酸处理组接受生理盐水和欧米伽3脂肪酸处理，欧米伽3脂肪酸+TAA组接受欧米伽3脂肪酸和TAA处理。TAA处理后3周和6周，分别检测大鼠体质量、肝重、生化指标，HE染色观察肝脏形态及组织结构。结果: TAA处理后3周和6周，会引起大鼠体质量增量显著下降，而肝脏重量/体质量比值显著增高。此外，血清丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶、谷氨酰转移酶和总胆红素水平显著提高。TAA处理3周后，肝脏组织切片显示形态学异常，特征是伴随细胞外基质胶原蛋白成分增多的明显的纤维化，以及肝细胞结构的损伤。TAA处理6周后，肝脏组织切片显示细胞外基质胶原蛋白成分增多，以及桥接纤维化形成。欧米伽3 脂肪酸能显著减轻TAA中毒大鼠组织病理学变化。结论: 欧米伽3 脂肪酸具有抗氧化作用，能够抵抗TAA引起的肝纤维化，可用于肝纤维化治疗。

关键词: 肝纤维化, 硫代乙酰胺, 欧米伽3 脂肪酸

Abstract:

AIM: To investigate the possible protective effect of omega-3 fatty acids (n-3 FAs) from fish oil on hepatic fibrosis induced by thioacetamide (TAA) in rats. METHODS: Sixty experimental animals were divided into four groups. The control group received normal saline solution. The TAA group was given 250 mg/kg body weight of TAA. The omega-3 fatty acids group was given saline solution and supplemented with n-3 FAs. The n-3 FAs + TAA group was treated with n-3 FAs and TAA. At the end of TAA treatment for three weeks and six weeks, body weight, liver weight, and biochemical indexes were detected. HE staining was used to observe liver morphology and organizational structure. RESULTS:Treatment of rats with TAA for three and six weeks resulted in a significant decrease in body weight, while the value of liver/body weight ratio was statistically increased. Furthermore, the levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase and total bilirubin were significantly increased. After three weeks of exposure to only TAA, liver sections showed an abnormal morphology characterized by noticeable fibrosis with the extracellular matrix collagen contents and damage of liver cells' structure. Liver sections from rats treated with only TAA for six weeks revealed an obvious increase in extracellular matrix collagen content and bridging fibrosis. Treating TAA-intoxicated rats with n-3 FAs significantly attenuated the severe physiological and histopathological changes.CONCLUSION: n-3 FAs have the antioxidation ability and can act against hepatic fibrosis induced by TAA, thus supporting its use in hepatic fibrosis therapy.

Key words: hepatic fibrosis, thioacetamide, omega-3 fatty acids

中图分类号:

R965.2
R575

曾熹，胡杨柳子，吴亚敏，陈志恒. 欧米伽3脂肪酸对硫代乙酰胺诱导的大鼠肝纤维化的保护作用[J]. 中国临床药理学与治疗学, 2017, 22(8): 892-898.

ZENG Xi, HUYANG Liuzi, WU Yamin, CHEN Zhiheng. Protective effect of omega-3 fatty acids on hepatic fibrosis of rats induced by thioacetamide[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(8): 892-898.

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