香菇多糖对卵白蛋白诱导小鼠哮喘模型树突细胞TIM4表达及炎症影响

doi:10.12092/j.issn.1009-2501.2018.09.005

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (9): 998-1002.doi: 10.12092/j.issn.1009-2501.2018.09.005

香菇多糖对卵白蛋白诱导小鼠哮喘模型树突细胞TIM4表达及炎症影响

陈叶¹，贺守第²，胡智立¹，冯斌¹

¹深圳市南山区人民医院儿科，²深圳市南山区人民医院中医风湿科，深圳 518000，广东

收稿日期:2018-05-14 修回日期:2018-06-25 出版日期:2018-09-26 发布日期:2018-09-26
作者简介:陈叶，女，硕士研究生，副主任医师，主要从事过敏性哮喘、儿童重症方面研究。 Tel：18666229677 E-mail：dytze2005@126.com
基金资助:
广东省科技计划项目（2017A020215129）；深圳市卫生计生科研项目（201506091）；深圳市南山区科技项目（2015021,2015043,2015047,2017016）；深圳市科技计划项目(JCYJ20160429183052202)

TIM4 expression of dendritic cells and inflammation effects in OVA-induced asthma model treated with lentinan

CHEN Ye¹, HE Shoudi², HU Zhili¹, FENG Bin¹

¹ Department of Pediatrics, Shenzhen Nanshan People's Hospital, Shenzhen 518000, China; ²Traditonal Chinese Medicine Department of Rheumatology, Shenzhen Nanshan People's Hospital, Shenzhen 518000, China

Received:2018-05-14 Revised:2018-06-25 Online:2018-09-26 Published:2018-09-26

摘要/Abstract

摘要：

目的: 研究香菇多糖治疗卵白蛋白（OVA）诱导小鼠哮喘模型后树突状细胞TIM4表达情况及炎症影响。方法: 将Balbc雌性小鼠随机分为三组：正常对照组、哮喘模型组、香菇多糖治疗组。OVA诱导激发小鼠哮喘模型，治疗组于第7天起每天进行香菇多糖腹腔注射治疗，哮喘模型组用生理盐水替代香菇多糖进行腹腔注射。分离脾脏单个核细胞，流式细胞学术检测树突细胞TIM4表达；ELISA检测血清IL-4和MCP-1浓度；Q-PCR检测肺组织中TIM4表达；肺组织HE染色。结果:治疗后，治疗组能下调脾脏树突细胞TIM4表达（P<0.05），降低血清中MCP-1、IL-4浓度（P<0.05，P<0.01）。肺组织中TIM4的RNA表达量下降（P<0.05）。HE染色显示治疗组肺组织炎症浸润明显减轻。结论: 香菇多糖能通过下调树突细胞TIM4表达，降低炎症因子IL-4、MCP-1等浓度，抑制OVA哮喘模型的炎症反应。

关键词: 香菇多糖, 哮喘, TIM4, 树突状细胞, MCP-1, OVA

Abstract:

AIM: To study the expression of TIM4 in dendritic cells and the effect of inflammation in OVA-induced asthma model mice treated with lentinan. METHODS: Balbc female mice were randomly divided into three groups: normal control group, asthma model group, lentinan treatment group. The asthma model was induced by OVA in mice. In the treatment group, lentinan was injected intraperitoneally every day from the 7th day, and the asthma model group was injected intraperitoneally with physiological saline instead of lentinan. The expression of TIM4 in dendritic cells was detected by flow cytometry in mononuclear cells from spleen. ELISA was used to detect the concentration of MCP-1 and IL-4 in the serum. Q-PCR was used to detect the expression of TIM4 in lung tissue and HE staining for lung. RESULTS: After treatment, lentinan could down-regulate the expression of TIM4 in dendritic cells from spleen (P<0.05), decrease the concentration of IL-4 and MCP-1in the serum (P<0.05, P<0.01). The expression of TIM4 RNA in lung tissue was decreased (P<0.05). HE staining showed that the inflammation of the lung tissue in the treatment group was significantly reduced. CONCLUSION: Lentinan can inhibit the expression of TIM4 in dendritic cells, reduce the concentrations of inflammatory cytokines such as IL-4 and MCP-1, and inhibit the inflammatory response in OVA-induced asthma model.

Key words: lentinan, asthma, TIM4, dendritic cells, MCP-1, OVA

中图分类号:

R965.2

陈叶，贺守第，胡智立，冯斌. 香菇多糖对卵白蛋白诱导小鼠哮喘模型树突细胞TIM4表达及炎症影响[J]. 中国临床药理学与治疗学, 2018, 23(9): 998-1002.

CHEN Ye, HE Shoudi, HU Zhili, FENG Bin. TIM4 expression of dendritic cells and inflammation effects in OVA-induced asthma model treated with lentinan[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(9): 998-1002.

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香菇多糖对卵白蛋白诱导小鼠哮喘模型树突细胞TIM4表达及炎症影响

TIM4 expression of dendritic cells and inflammation effects in OVA-induced asthma model treated with lentinan

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