miR-142-5p通过DNA甲基转移酶1抑制胃癌细胞增殖迁移的机制研究

doi:10.12092/j.issn.1009-2501.2019.12.002

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (12): 1328-1334.doi: 10.12092/j.issn.1009-2501.2019.12.002

miR-142-5p通过DNA甲基转移酶1抑制胃癌细胞增殖迁移的机制研究

李玉琴，王凯，敖丽，鲁晓岚，施建平

上海市浦东医院消化内科，上海 201399

收稿日期:2019-10-30 修回日期:2019-11-21 出版日期:2019-12-26 发布日期:2020-01-07
通讯作者: 施建平，男，本科，主任医师，研究方向：消化道肿瘤发病机制的研究。 Tel：18918790195 E-mail：18918790195@163.com
作者简介:李玉琴，女，硕士，主治医师，研究方向：长期从事胃癌发病机制的研究。 Tel：18019412513 E-mail：psckb65@163.com
基金资助:
上海市浦东新区卫生系统重点学科建设资助（PWZxk 2017-27）

miR-142-5p inhibits proliferation and migration of gastric cancer cells through DNMT1

LI Yuqin, WANG Kai, AO Li, LU Xiaolan, SHI Jianping

Department of Gastroenterology, Shanghai Pudong Hospital, Shanghai 201399, China

Received:2019-10-30 Revised:2019-11-21 Online:2019-12-26 Published:2020-01-07

摘要/Abstract

摘要：

目的:探究miR-142-5p在胃癌（GC）细胞中的作用并研究其机制。方法:qRT-PCR检测miR-142-5p表达；CCK-8和Transwell实验研究miR-142-5p对细胞增殖及迁移的影响；双荧光素酶报告基因和Western blot验证miR-142-5p的作用靶点。结果:miR-142-5p在GC组织及细胞系中的表达分别低于人正常胃组织及胃正常上皮黏膜细胞系GES-1，且其表达水平与胃癌肿瘤大小及淋巴结转移密切相关。在MKN28细胞中过表达miR-142-5p及在BGC-823细胞中沉默miR-142-5p能够分别抑制及促进胃癌细胞的增殖和迁移。DNA甲基转移酶1（DNMT1）是miR-142-5p直接作用靶点，miR-142-5p能够直接调节DNMT1的表达，并通过DNMT1调控胃癌细胞增殖及迁移。结论:miR-142-5p在GC中的表达降低，并在胃癌细胞中发挥抑癌基因的作用，能够通过抑制DNMT1来抑制胃癌细胞的增殖和迁移。

关键词: 胃癌, miR-142-5p, 增殖, 迁移, DNA甲基转移酶1

Abstract:

AIM: To investigate the role of miR-142-5p in gastric cancer（GC） cells and its mechanism. METHODS: The expression of miR-142-5p was detected by qRT-PCR; the effects of microRNA-142-5p on cell proliferation and migration were studied by CCK8 and Transwell assays; and the target of miR-142-5p was confirmed by dual luciferase reporter assay gene and Western blot assays. RESULTS:The expression of miR-142-5p in GC tissue and cell line was lower than that in normal gastric tissues and GES-1 cells, and the expression level of miR-142-5p was closely related to tumor size and lymph node metastasis. Overexpression of miR-142-5p in MKN28 cells and silencing miR-142-5p in BGC-823 cells inhibited and promoted the proliferation and migration of gastric cancer cells, respectively. DNA methyltransferase 1 (DNMT1) was the direct target of miR-142-5p, which could directly be regulated by miR-142-5p, and miR-142-5p regulated the proliferation and migration of gastric cancer cells through DNMT1. CONCLUSION: miR-142-5p is decreased in GC, implying an anti-oncogenetic effect to inhibit the proliferation and migration of gastric cancer cells by inhibiting DNMT1.

Key words: gastric cancer, miR-142-5p, proliferation, migration, DNA methyltransferase 1

中图分类号:

李玉琴，王凯，敖丽，鲁晓岚，施建平. miR-142-5p通过DNA甲基转移酶1抑制胃癌细胞增殖迁移的机制研究[J]. 中国临床药理学与治疗学, 2019, 24(12): 1328-1334.

LI Yuqin, WANG Kai, AO Li, LU Xiaolan, SHI Jianping. miR-142-5p inhibits proliferation and migration of gastric cancer cells through DNMT1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1328-1334.

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miR-142-5p通过DNA甲基转移酶1抑制胃癌细胞增殖迁移的机制研究

miR-142-5p inhibits proliferation and migration of gastric cancer cells through DNMT1

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