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中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (1): 57-60.

• 临床药理学 • 上一篇    下一篇

湖南汉族人群TNF-α基因-308G/A多态与原发性高血压相关性研究

彭翠英, 周翠兰, 贺庆芝   

  1. 南华大学药学与生命科学学院生物科学系,衡阳 421001,湖南
  • 收稿日期:2010-08-09 修回日期:2010-10-21 发布日期:2020-09-16
  • 作者简介:彭翠英,女,博士,副教授,研究方向:高血压相关研究。Tel: 0734-6200789 E-mail: pengcuiying2004@126.com
  • 基金资助:
    国家863基金项目(2007AA02Z4A7)

Association of TNF-α gene -308G/A polymorphism with essential hypertension in Han racial origin in Hunan

PENG Cui-ying, ZHOU Cui-lan, HE Qing-zhi   

  1. Pharmacy and Life Science, South China University, Hengyang 421001, Hunan,China
  • Received:2010-08-09 Revised:2010-10-21 Published:2020-09-16

摘要: 目的: 研究TNF-α基因-308G/A多态与原发性高血压(EH)的相关性。方法: 选择246例EH患者和208例正常对照组为研究对象,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法检测TNF-α基因启动子区-308G/A多态位点等位基因以及基因型频率分布特点,并分析其多态与EH遗传易感性的关系。结果: EH组患者TNF-α(-308G/A)位点GG、GA、AA三种基因型的频率分别为 85.0%、11.8%、3.2%;G、A两种等位基因的频率分别为 90.9%、9.1%。对照组TNF-α(-308G/A)位点GG、GA、AA三种基因型的频率分别为 89.4%、10.6%、0;G、A两种等位基因的频率分别为 94.7%、5.3%。EH组GA、AA基因型频率显著高于对照组(P=0.028);A等位基因的频率亦明显高于对照组,患高血压的危险性增加1.729倍,95%的可信区间为 1.056~2.831,差异均有统计学意义(P=0.030)。结论: TNF-α基因-308G/A位点多态与湖南汉族人群EH可能相关,A等位基因可能是EH的遗传易感基因。

关键词: 肿瘤坏死因子, 原发性高血压, 基因多态性, 遗传易感性

Abstract: AIM: To investigate the association TNF-α gene -308G/A polymorphism with essential hypertension(EH).METHODS: Polymerase chain reaction and Restriction fragment length polymorphism (PCR-RFLP) was used to screen the TNF-α -308G/A polymorphism and the distribution feature of genotypic frequency in 246 EH patients and 208 healthy people.The association of polymorphism and EH hereditary susceptibility was analysed.RESULTS: In the EH group, the frequencies of GG, GA and AA genotypes were 85.0%, 11.8% and 3.2% ,respectively. The frequencies of G and A allele were 90.9% and 9.1%,respectively. In the control group, the frequencies of GG, GA and AA genotypes were 89.4%, 10.6% and 0, respectively. The frequencies of G and A allele were 94.7% and 5.3%,respectively.Compared with the control group, the frequencies of GA , AA genotypes (P=0.028) and A allele were higher in the EH group.Compared with -308GG homozygotes,patients with the -308A allele had a 1.729-fold greater risk of developing EH, (95% confidence interval, 1.056 to 2.831). The differences were statistical significance (P=0.030).CONCLUSION: The TNF-a -308G/A polymorphism is positively associated with EH in Han racial origin in Hunan. The allele A may be a susceptibility gene of EH.

Key words: Tumor necrosis factor, Essential hypertension, Gene polymorphism, Hereditary susceptibility  ,  ,  ,  ,  

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