双黄连注射剂过敏症动物模型的研究

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (2): 154-157.

双黄连注射剂过敏症动物模型的研究

徐国良, 张卓辉, 张增珠, 张启云

江西中医学院现代中药制剂教育部重点实验室, 南昌 330004, 江西

收稿日期:2007-09-17 修回日期:2007-12-27 出版日期:2008-02-26 发布日期:2020-10-14
作者简介:徐国良, 男, 博士, 副教授, 硕士生导师, 主要从事中药药理的教学与科研。Tel:0791-7118657 　E-mail:xuguoliang6606@126.com
基金资助:
江西中医学院现代中药制剂教育部重点实验室主任基金资助

Experimental study to allergic animal models of Shuanghuanglian Injection

XU Guo-liang, ZHANG Zhuo-hui, ZHANG Zeng-zhu, ZHANG Qi-yun

Key Laboratory of Modern Preparation of TCM, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, Jiangxi, China

Received:2007-09-17 Revised:2007-12-27 Online:2008-02-26 Published:2020-10-14

摘要/Abstract

摘要： 目的:探索双黄连注射剂中引起Ⅰ 型过敏反应的致敏性物质, 建立双黄连注射剂过敏症动物模型。方法:将双黄连注射剂与正常兔血清体外孵育制备抗原, 免疫新西兰纯种兔, 制备抗双黄连注射剂中过敏性物质的抗血清, 并采用ELISA法检测其效价及抗体特异性。结果:双抗夹心ELISA 检测抗血清效价, 稀释至1:128 倍仍为阳性, 间接竞争ELISA 测定血清特异性并绘制抑制曲线, 得到回归方程I =13.362lgC +50.503, 相关系数r =0.966, 半数抑制浓度IC₅₀ =0.92 mg/mL。结论:此法初步建立了双黄连注射剂过敏症动物模型, 对双黄连注射剂中致敏物质的研究有一定意义。

关键词: 双黄连注射剂, 过敏症动物模型, 抗血清, ELISA

Abstract: AIM:To establish the allergic animal model of Shuanghuanglian Injection in order to explore the sensitizing substance caused type Ⅰ hypersensitivity by Shuanghuanglian Injection.METHODS:The Shuanghuanglian Injection and rabbit blood serum were used to incubate in vitro to prepare antigen, then the pure bleed Rabbits of New Zealand were immuned to prepare the antiserum of sensitizing substance in Shuanghuanglian Injection.And the potency and antibody specificity were detected by ELISA.RESULTS:A double sandwich ELISA was used to detect the antiserum potency, when the potency were 128 times diluted it was still positive.The indirect competitive ELISA was used to determine the serological specificity and the inhibitory curve was drawn.The regression equation was I =13.362lgC +50.503, the correlation coefficient(r) was 0.966 and the half-inhibition concentration(IC₅₀) was 0.92 mg/mL.CONCLUSION:A hypersensitivity animal model of Shuanghuanglian Injection was preliminary established, and can be used in investigation of the sensitizing substance in Shuanghuanglian Injection.

Key words: Shuanghuanglian Injection, hypersensitive animal model, antiserum, ELISA

中图分类号:

R965.1

徐国良, 张卓辉, 张增珠, 张启云. 双黄连注射剂过敏症动物模型的研究[J]. 中国临床药理学与治疗学, 2008, 13(2): 154-157.

XU Guo-liang, ZHANG Zhuo-hui, ZHANG Zeng-zhu, ZHANG Qi-yun. Experimental study to allergic animal models of Shuanghuanglian Injection[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(2): 154-157.

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