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中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (10): 1122-1126.

• 基础研究 • 上一篇    下一篇

灯盏细辛注射液对小鼠肝微粒体细胞色素 P450 含量的影响

许黎君1, 居文政2, 陈为烤1, 谈恒山3   

  1. 1南京中医药大学药学院, 南京 210029, 江苏;
    2南京中医药大学附属医院江苏省中医院临床药理实验室, 南京210029, 江苏;
    3南京军区总医院, 南京 210002, 江苏
  • 收稿日期:2008-07-24 修回日期:2008-09-25 出版日期:2008-10-26 发布日期:2020-10-19
  • 通讯作者: 居文政, 男, 博士, 主任中药师, 硕士生导师, 研究方向:中药药代动力学。Tel:025-86617141-80518  E-mail:njwz1008@jlonline.com
  • 作者简介:许黎君, 女, 硕士研究生, 研究方向:中药化学与分析。Tel:13770944591 E-mail:xulijun1203@163.com
  • 基金资助:
    科技部“十一五”支撑计划课题-药物临床研究关键技术研究与应用(2006BAI14B07)

Effects of the erigeron injection on liver microsomal cytochrome P450 system in mice

XU Li-jun1, JU Wen-zheng2, CHEN Wei-kao1, TAN Heng-shan3   

  1. 1College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu, China;
    2D-epartment of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nan-jing 210029, Jiangsu, China;
    3General Hospital of Armed Forces of Nanjing, Nanjing 210002, Jiangsu, China
  • Received:2008-07-24 Revised:2008-09-25 Online:2008-10-26 Published:2020-10-19

摘要: 目的: 研究灯盏细辛注射液对小鼠肝微粒体细胞色素P450含量的影响 。方法: 小鼠分为空白对照组 、阳性对照组(苯巴比妥钠 80 mg/kg, i .p ., 共 7 d) 、灯盏细辛注射液正常剂量组 、高剂量组(10、30 mg/kg, i .p., 共 14 d) 。用紫外分光光度法测定细胞色素 P450及其同工酶的活性 。结果: 灯盏细辛注射液两剂量组小鼠肝微粒体的蛋白含量和细胞色素 P450 含量较空白对照组增高, 对肝重指数基本无影响 ;可抑制红霉素-N-脱甲基酶(ERD) 的活性(P <0.01) , 而对氨基比林-N-脱甲基酶(AMD) 基本无影响(P >0.05) ;苯巴比妥钠组肝重指数、蛋白含量和细胞色素 P450 含量与其他各组比较均升高(P <0.05) , 对氨基比林-N-脱甲基酶 、红霉素-N-脱甲基酶活性均有诱导作用(P <0 .01) 。结论: 灯盏细辛注射液对小鼠肝微粒体蛋白、细胞色素 P450 含量有一定影响, 对CYP3A 可能有抑制作用, 对氨基比林-N-脱甲基酶活性基本无影响。

关键词: 灯盏细辛注射液, 小鼠, 肝微粒体, 细胞色素 P450

Abstract: AIM: To investigate the effects of eri-geron injection on liver microsomal cytochrome P450 (CYP450) system in mice .METHODS: Mice were divided into control group (CG) , positive control group, normal-dosage group (NG) and high-dosage group (HG) .The mice of control group were given sa-line only and the mice of positive control group were given Phenobarbital sodium injection (PBN) at 80 mg/kg by peritoneal injection (i .p.) , once daily for seven days.The remaining two groups were i .p.with 10 mg/kg (normal-dosage group) and 30 mg/kg (high-dosage group) erigeron injection, respectively, once daily for two weeks.Then the total content and activi-ties of CYP450 of mice were determined by spectropho-tometer .RESULTS: The study showed that the cont-ent of CYP450 and protein in mice of normal-dosage group and high-dosage group were higher than those in control group, except liver indexes.In terms of the eri-geron injection effects on Erythromycin-N-demethylase and aminopyrine-N-demethylase, the normal-dosage group and high-dosage group can decrease the activity of Erythromycin-N-demethylase (P <0.01) , but had no effect on aminopyrine-N-demethylase (P >0.05) . Phenobarbital sodium can increase the levels of liver indexes, the content of CYP450 and protein(P < 0.05) , Erythromycin-N-demethylase and aminopyrine-N-demethylase (P <0.01) .CONCLUSION: The erigeron injection had no effect on the liver indexes and the levels of aminopyrine N-demethylase in mice. However, the content of CYP450 and protein, and the levels of Erythromycin N-demethylase were affected. The erigeron injection may be an inhibitive agent to CYP3A.

Key words: erigeron injection, mice, liver micro-some, cytochrome P450

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