粉防己碱促进和敏化肾性高血压大鼠血管平滑肌细胞凋亡

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (4): 412-416.

粉防己碱促进和敏化肾性高血压大鼠血管平滑肌细胞凋亡

张莉, 张长平, 李庆平

南京医科大学药理学系, 南京210029, 江苏

收稿日期:2007-03-06 修回日期:2007-03-06 发布日期:2020-10-30
通讯作者: 李庆平,女, 博士, 教授, 硕士生导师, 研究方向:心血管药理学。Tel:025-86862883 E-mai l:qpli @njmu.edu.cn
基金资助:
江苏省自然科学基金(BK97049); 国家自然科学基金(30271506)

Tetrandrine induces and sensitizes vascular smooth muscle cell apoptosis in renovascular hypertensive rats

ZHANG Li, ZHANG Chang-ping, LI Qing-ping

Department of Pharmacology, Nanjing Medical University, Nanjing 210029, Jiangsu, China

Received:2007-03-06 Revised:2007-03-06 Published:2020-10-30

摘要/Abstract

摘要： 目的从大鼠血管平滑肌细胞凋亡角度探讨粉防己碱逆转肾血管性高血压血管重构的机制。方法两肾一夹法制备肾性高血压模型, 原位末端标记法检测主动脉和尾动脉动脉壁平滑肌细胞凋亡数量;[3H]-TdR 掺入和流式细胞术分析粉防己碱对培养细胞凋亡率的影响。结果粉防己碱能够诱导血管平滑肌细胞凋亡, 并使低血清和TNFα、转化生长因子(TGFβ1) 诱导的细胞凋亡率增加。结论粉防己碱逆转肾性高血压大鼠(RHR) 血管重构的机制可能与诱导和敏化RHR 血管平滑肌细胞凋亡有关。

关键词: 肾血管性高血压, 血管重构, 血管平滑肌细胞, 细胞凋亡, 粉防己碱

Abstract: AIM: To determine whether apoptosis is involved in the development of vascular remodeling in renovascular hypertension rats and whether the inhibitory effect of tetrandrine on proliferation is mediated by apoptosis. METHODS: Apoptosis was detected in histologic sections of aorta and tail arteries with in situ end-labeling.The apoptosis ratio in cultured cells was measured by [3H]-TdR incorporation and flow cytometer analysis method. RESULTS: Compared with the sham group, the apoptosis rate of renal hypertension rat (RHR) increased. Tetrandrine (Tet) directly induced apoptosis in cultured AVSMCs (from RHR) and increased apoptosis induced by TGFα, TGFβ1 and deficient FCS. CONCLUSION: Tet induces and sensitizes vascular smooth muscle cells of RHR to apoptosis, which at least partly related to the regression of vascular remodeling.

Key words: renovascular hypertension, vascular remodeling, vascular smooth muscle cell, apoptosis, tetrandrine

中图分类号:

张莉, 张长平, 李庆平. 粉防己碱促进和敏化肾性高血压大鼠血管平滑肌细胞凋亡[J]. 中国临床药理学与治疗学, 2007, 12(4): 412-416.

ZHANG Li, ZHANG Chang-ping, LI Qing-ping. Tetrandrine induces and sensitizes vascular smooth muscle cell apoptosis in renovascular hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(4): 412-416.

参考文献

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