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中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (3): 287-290.

• 基础研究 • 上一篇    下一篇

毛细管电色谱手性配体交换法检测生物样品中的硝基精氨酸异构体研究

辛艳飞, 李丽丽, 马爱妞, 伍媚, 周向军, 王永祥   

  1. 上海交通大学药学院分子药理实验室, 上海200240
  • 收稿日期:2006-10-17 修回日期:2007-02-09 出版日期:2007-03-26 发布日期:2020-11-06
  • 通讯作者: 王永祥, 男, 博士, 教授, 博士生导师, 研究方向:药理学。Tel:021-34204763 E-mail:yxwang@sjtu.edu.cn.
  • 作者简介:辛艳飞, 男, 博士, 助理研究员, 研究方向:手性药理与药物代谢。Tel:0571-88215633 E-mail:xinyanfei@gmail.com
  • 基金资助:
    国家自然科学基金资助项目(30472052)

Determination of NG-nitro-arginine enantiomers in biological samples with chiral ligand exchange method by capillary electrochromatography

XIN Yan-fei, LI Li-li, MA Ai-niu, WU Mei, ZHOU Xiang-jun, WANG Yong-xiang   

  1. Laboratory of Molecular Pharmacology, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2006-10-17 Revised:2007-02-09 Online:2007-03-26 Published:2020-11-06

摘要: 目的:建立毛细管电色谱(CEC) 分离检测生物样品中硝基精氨酸异构体的方法。方法:采用CEC 手性配体交换模式检测血浆样品中D-硝基精氨酸(D-NNA) 和L-硝基精氨酸(L-NNA), 流动相为50 mmol/L 醋酸缓冲液[pH 5.0, 含2 mmol/L aspartame,1 mmol/L Cu2+ 和5 % (v v) 甲醇];流速为0.02 mL/min;操作压为1 000 psi;检测波长为UV 280 nm 。结果:L-NNA 和D-NNA 在0.025 ~0.75 mmol/L 的浓度范围内峰面积 浓度的相关系数均可达0.99 以上, 日内变异系数均小于3.0 %, 日间变异系数分别为3.1 %和3.4 %。结论:本研究方法具有快速、高分辨、检测样品量和流动相用量少等优点。

关键词: 硝基精氨酸, 手性拆分, 手性配体交换法, 毛细管电色谱, 血浆

Abstract: AIM: To study the chiral separation of NG-nitro-arginine enantiomers in biological samples by capillary electrochromatography (CEC).METHODS: The separation of D-NNA and L-NNA in plasma was achieved by chiral ligand exchange method via CEC.An aqueous eluent containing aspartame (2 mmol/L) sufficiently mixed with Cu2+ was used.The eluent was monitored at UV 280 nm.RESULTS: The retention times were 16 and 19 min for L-NNA and D-NNA, respectively.The standard curves of D-NNA and L-NNA ranged from 0.025 to 0.75 mmol/L (R2 >0.98).The withinday precision of the quality control samples was 3.0 %for both L-NNA and D-NNA.The between-day precision was 3.1 % for L-NNA and 3.4 % for D-NNA, respectively.CONCLUSION: This method was established to determinate D L-NNA in biological samples with high resolution and efficiency.

Key words: NG-nitro-arginine, chiral separation, ligand exchange, capillary electrochromatography, plasma

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