非神经性 AChRs 对脾淋巴细胞增殖的调节作用及药理学特征

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (11): 1304-1308.

非神经性 AChRs 对脾淋巴细胞增殖的调节作用及药理学特征

温晓明^1,2, 张颖丽², 刘新民¹, 郭顺星¹, 汪海²

¹中国协和医科大学 & 中国医学科学院药用植物研究所,北京 100094;
²军事医学科学院毒物药物研究所,北京 100850

收稿日期:2005-09-29 修回日期:2005-10-31 出版日期:2005-11-26 发布日期:2020-11-12
通讯作者: 汪海,男, 博士, 研究员, 研究方向:分子药理学和心血管药理学。Tel:010-66982651 E-mail:wh9588@yahoo.com.cn
基金资助:
自然科学基金项目(No30371641);军事医学科学院创新基金项目

Effects of non-neuronal AChRs on proliferation of splenocytes of mice

WEN Xiao-ming^1,2, ZHANG Ying-li², LIN Xi-min¹, GUO Shun-xing¹, WANG Hai²

¹Institute of Medicinal Plant Development,CAMS & PUMC,Beijing 100094,China;
²Institute of Pharmacology and Toxi-cology,Academy of Military Medical Sciences,Beijing 100850,China

Received:2005-09-29 Revised:2005-10-31 Online:2005-11-26 Published:2020-11-12

摘要/Abstract

摘要： 目的: 观察淋巴细胞非神经性AChRs对脾淋巴细胞增殖的调节作用。方法: 利用MTT比色法检测AChRs激动剂氨甲酰胆碱对刀豆蛋白A诱导的离体小鼠脾淋巴细胞增殖反应的影响。结果: 氨甲酰胆碱在不同的浓度呈现不同的作用,在10^-11~10^-9mol·L^-1和10^-5~10^-4mol·L^-1时显著促进淋巴细胞的增殖,阿托品(10^-7mol·L^-1)可以阻断该效应。而氨甲酰胆碱(10^-7~10^-6mol·L^-1)可显著抑制淋巴细胞的增殖,美加明(10^-7mol·L-1)可以阻断其作用。提示氨甲酰胆碱在10^-11~10^-9mol·L^-1和10^-5~10^-4mol·L^-1可通过激活mAChRs促进淋巴细胞的增殖,而在10^-7~10^-6mol·L^-1通过激活nAChRs抑制淋巴细胞的增殖。结论: 氨甲酰胆碱通过激活非神经性mAChRs和nAChRs对淋巴细胞增殖反应产生促进增殖和抑制增殖的双向调节作用。

关键词: 非神经性, AChRs, 淋巴细胞, 增殖反应, 氨甲酰胆碱

Abstract: AIM: To examine the effects of non-neu-ronal AChRs on the proliferation of splenocytes.METHODS: The effects of AChRs agonist carbachol on the prolif-eration of mice splenocytes stimulated by concanavalin measured by MTT reduction assay in vitro.RESULTS: The splenocytes responded to carbachol(in the range of 10^-11-10^-9mol·L^-1and 10^-5-10^-4mol·L^-1)byin-creased proliferation which were blocked by the muscarin-ic antagonist atropine(10^-7mol·L^-1).In contrast,car-bachol(10^-7-10^-6mol·L^-1)produced diminished pro-liferation,an effect which were blocked by the nAChRs antagonist mecamylamine(10^-7mol·L^-1).It suggests that mAChRs was sensitive to 10^-11-10^-9mol·L^-1and 10^-5-10^-4mol·L^-1carbachol,resulting in increasing proliferation and nAChRs responses to 10^-7~ 10^-6mol·L^-1carbachol leading to proliferation inhibition.CONCLUSION: Carbachol can elicit two different ef-fects according to different concentrations in splenocytes via non-neuronal mAChRs or nAChRs.

Key words: non-neuronal, ACh receptors, lympho-cytes, proliferation, carbachol

中图分类号:

R965.1

温晓明, 张颖丽, 刘新民, 郭顺星, 汪海. 非神经性 AChRs 对脾淋巴细胞增殖的调节作用及药理学特征[J]. 中国临床药理学与治疗学, 2005, 10(11): 1304-1308.

WEN Xiao-ming, ZHANG Ying-li, LIN Xi-min, GUO Shun-xing, WANG Hai. Effects of non-neuronal AChRs on proliferation of splenocytes of mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(11): 1304-1308.

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