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中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (6): 689-691.

• 研究原著 • 上一篇    下一篇

参麦注射液对自由基所致兔离体心脏功能损伤的保护作用

李年生, 胡长平   

  1. 中南大学药学院药理学系,长沙 410078,湖南
  • 收稿日期:2003-06-09 修回日期:2003-07-10 出版日期:2003-12-26 发布日期:2020-11-19
  • 通讯作者: 胡长平,男,博士,研究方向:心血管药理学。Tel:0731-4805441  Fax:0731-4471289 E-mail:huchangping2001@yahoo.com.cn
  • 作者简介:李年生,男,大学,主管实验师,研究方向:心血管药理学。

Protective effects of shenmai injection on free radical-induced myocardial injury in isolated rabbit hearts

LI Nian-Sheng, HU Chang-Ping   

  1. Department ofPharmacology,School ofPharmaceutical Sciences,Central SouthUniversity,Changsha 410078,Hunan,China
  • Received:2003-06-09 Revised:2003-07-10 Online:2003-12-26 Published:2020-11-19

摘要: 目的 在兔离体灌流心脏观察参麦注射液对1,1-二苯-2-三硝基苯肼(DPPH)自由基所致心功能损伤的保护作用。方法 离体兔心脏采用Langendorff法灌流,记录左室内压(LVP)、左室内压最大上升速率(+dp/dtmax)、左室舒张末压(LVEDP)、心率(HR),定时收集冠脉流出液测量冠脉流量(CF)和肌酸激酶(CK)活性。结果 用含0 .25μmol·L-1DPPH的K-H 液灌流心脏10 min,可显著降低LVP、+dp/dtmax,升高LVEDP,减慢HR,增加CK 释放;用含参麦注射液(1∶320 、1∶160)的K-H 液灌流心脏10 min,可明显促进DPPH 所致心功能损伤的恢复,减少CK 的释放。结论 参麦注射液对DPPH 自由基所致心功能损伤具有明显保护作用。

关键词: 药理学, 参麦注射液, 1,1-二苯-2-三硝基苯肼(DPPH), 自由基, 心脏,

Abstract: AIM: To observe theProtective effects of shenmai injection on myocardial injury induced by 1,1-dipheyl-2-picryl hydrazyl(DPPH)free radical in isolated rabbit hearts.METHODS: The isolated rabbit hearts werePerfused in alangendorff model.Left ventricularPressure(lVP),the first derivative oflVP(+dp/d tmax),left ventricular end-diastolicPressure(lVEDP)and heart rate(HR)were recorded.Coronary flow(CF)and the activity of creatine kinase(CK)in coronary effluent were measured by timed collection of coronary effluent.RESULTS:Perfusion with DPPH(0.25μmol·L-1)for 10 min caused a significant impairment of cardiac function,as shown by a decrease inlVP,+dp/dtmax and HR and an increase inlVEDPas well as the increased release of CK.AfterPerfusion with DPPH(0.25μmol·L-1)for 10min,treatment with shenmai injection(1∶320 or 1∶160)for 10minProduced a significant improvement of cardiac function and a decrease in the release of CK.CONCLUSION: Shenmai injectionProtects against myocardial injury induced by DDPH free radical in isolated rabbit hearts.

Key words: pharmacology, shenmai injection, DPPH, free radical, heart, rabbit

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