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中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (6): 515-517.

• 研究原著 • 上一篇    下一篇

蝎毒组分Ⅲ对人头颈部鳞状细胞癌细胞生长的影响

卢娜, 王辉1, 杨献军2, 李超3   

  1. 新乡医学院生理学教研室,1免疫学教研室,2分子生物学研究室,3三附院眼科,新乡 453003
  • 收稿日期:2002-08-16 修回日期:2002-08-26 出版日期:2002-12-26 发布日期:2020-11-20
  • 通讯作者: 卢娜,女,硕士研究生,研究方向:生物毒素对肿瘤的作用。Tel:0373-3063496  E-mail:luna1975@etang.com

Effect of the component Ⅲ in scorpion venom crude extracted from buthus martensii karsch on the human jugular squamous cell carcinoma AGZY-973cell line

LU Na, WANG Hui1, YANG Xian-Jun2, LI Chao3   

  1. Department of Physiology,1Department of Immunology,2Molecule Biology Laborator,3Department of Ophthalmology ofthe Third Affiliated Hospital,Xinxiang Medical College,Xinxiang 453003
  • Received:2002-08-16 Revised:2002-08-26 Online:2002-12-26 Published:2020-11-20

摘要: 目的 观察蝎毒组分Ⅲ(SVC-Ⅲ)对人头颈部鳞状细胞癌细胞株(AGZY-973)生长的影响,试图找出SVC Ⅲ抑制鳞状细胞癌的最佳浓度。方法 采用噻唑蓝(MTT)法检测经不同浓度SVC-Ⅲ 作用下AGZY-973 生长抑制率。结果 SVC Ⅲ能抑制该细胞的生长,SVC-Ⅲ可导致AGZY-973 细胞代谢MTT 的能力显著低于对照组,生长抑制率达68.96%(P <0.01)。当SVC Ⅲ的浓度<40 mg·L-1时,剂量效应关系明显;而SVC-Ⅲ的浓度>40 mg·L-1时无剂量效应关系。5-Fu 治疗组生长抑制率达55.27% P <0.01)。结论 SVC-Ⅲ是存在于东亚钳蝎蝎毒中的一种抗肿瘤成分,能够抑制AGZY-973 的生长,并在一定浓度范围内存在剂量效应关系。

关键词: 蝎素, 细胞株, 鳞状细胞癌, 细胞毒性, 抗肿瘤

Abstract: AIM: To observe the cytotoxicity effect of scorpion venom crude(SVC-Ⅲ)on the human jugular squamous cell carcinoma(AGZY-973)and to find a properly consistence of SVC of the growth inhibiting to AGZY-973.METHODS: MTT colorimetric method was used to study the growth inhibition rate(GIR)in different condition.RESULTS: The ability to metabolize MTT of AGZY-973 cells was lower than control distinctly after the cells were treated by SVC-Ⅲ.The growth of AGZY-973 cells was inhibited obviously by SVC Ⅲ and GIR was reached 68.96%(p<0.01).The dose-response relationshipwas unclear when consistence were more than 40mg·L-1,while the dose-response relationshipwas clear when dose was in 10 -40 mg·L-1.The growth inhibition rate(GIR)of 5-Fu reached 55.27%(p<0.01).CONCLUSION: SVC is one kind of effective antineoplastic components in the venom of buthus martensii scorpion.It possesses growth inhibition on AGZY-973 cells.The properly consistence of SVC of the growth inhibition to AGZY-973 cells probably is 40 mg·L-1.

Key words: scorpion venom, cell line, squamous cell carcinoma, cells cytotoxicity, antitumor

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