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中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (3): 282-285.

• 研究原著 • 上一篇    下一篇

一氧化氮与光化学法诱导大鼠脑缺血早期损伤的关系和绞股蓝总皂苷对脑缺血损伤的保护作用1

谢志忠, 朱炳阳, 唐小卿, 廖端芳, 余麟   

  1. 南华大学药物药理研究所, 衡阳 421001, 湖南
  • 收稿日期:2002-12-06 修回日期:2003-01-08 出版日期:2003-06-26 发布日期:2020-11-25
  • 通讯作者: 谢志忠, 男, 硕士, 讲师, 研究方向:心脑血管疾病机制及药物干预。Tel:0734-8281408 E-mail:zhizhongx@hotmail.com
  • 作者简介:廖端芳, 男, 教授, 博士生导师, 研究方向:心脑血管疾病机制及药物干预。
  • 基金资助:
    1 湖南省自然科学基金资助项目(№;9420933); 湖南省教育厅课题基金资助项目(№;1191206)

Relationship between nitric oxide and early ischemic brain damage induced by photochemistry in rats and the protective effects of gypenosides on the damage1

XIE Zhi-Zhong, ZHU Bing-Yang, TANG Xiao-Qing, LIAO Duang-Fang, YU Lin   

  1. Institute of Pharmacy &Pharmacology, Nanhua University, Hengyang 421001, Hunan
  • Received:2002-12-06 Revised:2003-01-08 Online:2003-06-26 Published:2020-11-25

摘要: 目的: 探讨一氧化氮(NO) 与光化学法诱导大鼠脑缺血早期损伤的关系和绞股蓝总皂苷(Gyp) 对脑缺血损伤的保护作用。方法: 应用光化学法诱导大鼠大脑中动脉栓塞(MCAO) 模型, 观察氧合血红蛋白(OHb) 和Gyp 对MCAO 后脑梗死范围, 脑组织含水量、NO 及超氧化歧化酶(SOD)、脂质过氧化产物TBARS 含量的影响。结果: MCAO 6 h 可导致大鼠局部脑组织明显梗死、脑水肿, 同时SOD 活力下降, TBARS 含量升高, NO 释放减少。OHb 能进一步强化上述改变而Gyp 能逆转上述变化。结论: 脑内过氧化反应水平增高导致早期NO 含量减少是脑缺血形成的重要原因之一, Gyp 可通过影响脑缺血损伤早期脑内NO 水平发挥对脑缺血的保护作用。

关键词: 药理学, 绞股蓝总皂苷, 光化学反应, 脑缺血性损害, 一氧化氮

Abstract: AIM: To investigate the relationship between nitric oxide (NO) and early ischemic brain damage induced by photochemistry and the effect of gypenosides (Gyp) on the damage. METHODS: The effects of Gyp and oxidized hemoglobin (OHb) on ischemic brain tissure areas, TBARS and H2O contents, superoxide dismutase (SOD) activity and NO level were observed on the rat middle cerebral artery occlusion (MCAO) model induced by photochemistry. RESULTS: The middle cerebral artery occlusion for 6 h caused obvious ischemic brain damage, increased the areas of ischemic brain and the contents of H2O and TBARS, and decreased SOD activity and NO level (all P <0.05).These effects can be exacerbated seriously by OHb, but reversed by Gyp. CONCLUSION: The decrease of early cerebral NO level is an important factor to lead to ischemic brain damage.Gyp decreases ischemic brain damage by increasing early cerebral NO level and has a prophylactic protective effect on cerebral ischemic damage.

Key words: pharmacology, gypenosides, photochemistry, ischemic brain damage, nitric oxide

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