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中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (3): 295-298.

• 研究原著 • 上一篇    下一篇

中药复方重肌灵免疫调节作用及其机制的实验研究

韩涛, 周勇, 王旭丹, 葛东宇, 王静, 吴以岭1, 田书彦1, 高学东1, 李萍1   

  1. 北京中医药大学中西医结合免疫室, 北京 100029; 1河北以岭医药研究院, 石家庄 050091, 河北
  • 收稿日期:2002-11-13 修回日期:2003-01-06 出版日期:2003-06-26 发布日期:2020-11-25
  • 通讯作者: 韩涛, 男, 北京中医药大学2000 级博士。Tel:010-64286757 E-mail:htht8@163.com
  • 作者简介:周勇, 男, 教授, 博士生导师, 主要从事免疫学研究。吴以岭, 教授, 河北以岭医药研究院总裁。

Experimental study on immunoregulation mechanism of compound zhongjiling

HAN Tao, ZHOU Yong, WANG Xue-Dan, GE Dong-Yu, WANG Jing, WU Yi-Ling1, TIAN Shu-Yan1, GAO Xue-Dong1, LI Ping1   

  1. Beijing University of Chinese Medicine, Beijing 100029; 1Yiling Medical and Pharmacological Institute, Shijiazhuang 050091, Hebei
  • Received:2002-11-13 Revised:2003-01-06 Online:2003-06-26 Published:2020-11-25

摘要: 目的: 探讨中药复方重肌灵免疫调节作用及其机理。方法: 建立Lewis 大鼠EAMG 模型。采用3H-TdR 法检测重肌灵对ConA、脂多糖(LPS) 诱导正常BALB/C小鼠脾淋巴细胞增殖反应的影响, 及重肌灵对ConA、N2 AchR 诱导EAMG 大鼠淋巴结细胞增殖反应的影响。ELISA 法测定重肌灵对EAMG 大鼠血清中N2AchRab 滴度及血清中IFN-γ水平的影响。结果: 重肌灵对ConA、LPS 诱导正常ALB C 小鼠脾淋巴细胞增殖反应有明显的促进作用(P <0.05);对ConA 诱导EAMG 大鼠淋巴结细胞增殖反应有明显的促进作用(P <0.01), 而对N2AchR 诱导EAMG 大鼠淋巴结细胞增殖反应有明显抑制作用(P <0.01)。重肌灵对EAMG 大鼠血清中N2AchRab滴度及血清IFN-γ水平有明显下调作用(P <0.01)。结论: 重肌灵防治EAMG 不是通过免疫抑制, 而是利用它的免疫调节作用。机理可能是通过下调IFN-γ水平, 而抑制N2AchR 抗原诱导特异性免疫反应, 而起到治疗作用。

关键词: 药理学, 重肌灵, EAMG, 免疫调节, IFN-γ

Abstract: AIM: To approach the immunoregulation function and mechanism of compound zhongjiling. METHODS: EAMG models in Lews rats were established.By using 3H-TdR method, the effect of zhongjiling to proliferation reaction of lymphocyte in normal BALB/C spleen of mice induced by ConA and Lps and the effect of compound zhongjiling to proliferation reaction of lymphocyte in lymph node of rats was examined;by using ELISA, the effect of zhongjiling to AchRab titre and the level of IFN-γin serum of EAMG rats was examined. RESULTS: there was no significant improvement on the effect of zhongjiling to proliferation reaction of lymphocyte in normal BALB C spleen of mice induced by ConA and LPS(P <0.05).The effect of compound zhongjiling on the proliferation reaction of lymphocyte showed a significant improvement (P <0.01) in lymph node of EAMG rats induced by ConA, and the inhibition was induced by N2AchR (P <0.01).The AchRab titre and the level of IFN-γ significantly reduced (P <0.01) in serum of EAMG rats. CONCLUSIONs: The mechanism of compound zhongjiling is not through immunosuppression, but through restraining N2AchR antigen to induce specific immune reaction in prevention and treatment of EAMG.

Key words: pharmacology, compound zhongjiling, EAMG, immunoregulation, IFN-γ

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