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中国临床药理学与治疗学 ›› 1997, Vol. 2 ›› Issue (1): 37-39.

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阿魏酸钠对培养近端肾小管细胞庆大霉素毒性保护机理探讨

黄云剑, 瘳立生   

  1. 第三军医大学新桥医院肾内科,重庆 630037
  • 收稿日期:1996-12-30 修回日期:1997-01-28 出版日期:1997-03-26 发布日期:2020-12-04
  • 作者简介:黄云创,男,32岁,主治医师。硕士。研究方向中毒性肾病。

Protective mechanism of sodium ferulate against gentamicin - induced nephrotoxicity in Renal tubular cells

Huang Yunjian, Liao Lisheng   

  1. Department of Nephrology, Xinqiao Hospital, Third Military Medical University, Chongqing 630037
  • Received:1996-12-30 Revised:1997-01-28 Online:1997-03-26 Published:2020-12-04

摘要: 目的 研究阿魏酸钠(SF)对庆大霉素(GM)肾毒性的保护作用。方法 采用近端肾小管上皮细胞原代培养技术制作GM损伤模型,观察细胞丙氦酸氦基转换酶(ALT)、过氧化氢酶(Cat)、NAG酶活性,DNA合成,细胞内离子钙浓度以及形态学变化,同时观察SF的保护作用。结果 GM组表现为ALT,Cat酶活性下降,NAG酶活性增加,细胞DNA合成下降,胞内游离钙浓度升高。光镜发现近端小管上皮细胞内有大量空泡变性和溶酶体髓样体形成。给予SF能使上述异常指标明显改善,组织学损害也明显减轻。结论 SF能有效保护GM对肾小管上皮细胞的损害。

关键词: 阿魏酸钠, 庆大霉素, 肾毒性, 细胞培养

Abstract: Aim To investigate the effect of sodium ferulate(SF), a compound derived from Chinese herbs,on gentamicin (GM)nephrotoxicity in renal tubular cells.Methods Alanine aminopeptidase (ALT), catalase (Cat), and N-acetyl-β- glucosaminidase (NAG)activities, DNA synthesis, cytosolic free calcium concentration, and morphological alterations were observed in gentamicin-induced damage to cultured primary renal proximal tubular cells.The protection of SF to damages was observed too.Results In GM group, ALT and Cat activities decreased, NAG activity invreased;besides, DNA synthesis decreased and cytosolic free calcium concentration increased.Electronmicrosope observation revealed an extensive vacuolization in proximal tubular cells and formation of myeloid bodies in lysosome.Simultaneous administration of SF with GM could markedly improve the values of parameters mentioned above and the histological damages.Conclusion SF can protect renal proximal tubular cell from GM nephrotoxicity.

Key words: Sodium ferulate, Gentamicin, Nephrotoxicity Cell, Culture

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