药用注射辅料聚山梨酯80诱发类过敏反应的细胞研究

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (5): 501-505.

药用注射辅料聚山梨酯80诱发类过敏反应的细胞研究

李钦, 赵吟, 郑晓亮, 张信岳

浙江省医学科学院药物研究所, 杭州 310000, 浙江

收稿日期:2011-03-09 修回日期:2011-03-22 发布日期:2011-07-08
通讯作者: 张信岳,男,副研究员,硕士生导师,研究方向:新药临床前药理学评价及中药注射剂不良反应机制研究。Tel:0571-88215600 E-mail:zhangxy2003@yahoo.cn
作者简介:李钦,女,研究生,助理研究员,研究方向:新药临床前药理学评价及中药注射不良反应机制研究。E-mail:qindai1981@sohu.com
基金资助:
浙江省中医局青年基金(2008YA013)

Cell research on anaphylactoid reaction induced by pharmaceutical excipients Polysorbate 80

LI Qin, ZHAO Yin, ZHENG Xiao-liang, ZHANG Xin-yue

Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, Zhejiang,China

Received:2011-03-09 Revised:2011-03-22 Published:2011-07-08

摘要/Abstract

摘要： 目的: 观察聚山梨酯80诱导大鼠嗜碱性粒细胞白血病细胞株RBL-2H3细胞脱颗粒现象,探讨其诱发类过敏反应的可能机制。方法: 聚山梨酯80与RBL-2H3细胞共同孵育 60 min 后,透射电镜观察细胞发生脱颗粒反应的形态学变化,荧光显微镜下观察细胞膜磷脂酰丝氨酸与Annexin V结合变化,分光光度法测定细胞上清液β-氨基己糖苷酶释放百分率,并通过阿利新蓝染色试验进行定性观察。结果: 不同浓度聚山梨酯80可诱导细胞发生典型的脱颗粒反应形态学变化,与阴性对照组相比脱颗粒率及组胺释放率显著升高,且具有浓度依赖性。结论: 聚山梨酯80单次、首次给药即可诱导RBL-2H3细胞脱颗粒,释放组胺,诱发类过敏反应,这可能是其临床首次用药即引起严重不良反应的机制之一。

关键词: 药用辅料, 聚山梨酯80, RBL-2H3细胞, 脱颗粒, 类过敏反应

Abstract: AIM: To observe the effects of Polysorbate 80 on basophilic cell leukemia RBL-2H3 cell degranulation and discuss the possible mechanism of anaphylactoid reaction.METHODS: RBL-2H3 cells were incubated with Polysorbate 80 for 60 min, and then the morphological changes of cells degranulation were observed by transmission electron microscopy, cell degranulation rate was detected by Alcian blue dye assay, Annexin V binding assay and β-hexosaminidase assay.RESULTS: Different concentrations of Polysorbate 80 can induce the typical morphological changes in RBL-2H3 cell with degranulation.Compared with the control group,the rates of degranulation and histamine release in Polysorbate 80 treated cells were significantly increased and dose-dependent.CONCLUSION: RBL-2H3 cell degranulation,histamine can be induced by single dose Polysorbate 80, and then induced anaphylactoid reaction, which may be one of the possible mechanisms of serious adverse induced by Polysorbate 80 injection for the first administration in clinic.

Key words: Pharmaceutical excipients, Polysorbate 80, RBL-2H3 cell, Degranulation, Anaphylactoid reaction

中图分类号:

R965.2

李钦, 赵吟, 郑晓亮, 张信岳. 药用注射辅料聚山梨酯80诱发类过敏反应的细胞研究[J]. 中国临床药理学与治疗学, 2011, 16(5): 501-505.

LI Qin, ZHAO Yin, ZHENG Xiao-liang, ZHANG Xin-yue. Cell research on anaphylactoid reaction induced by pharmaceutical excipients Polysorbate 80[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(5): 501-505.

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