扬子鳄大脑胚后发育中一氧化氮合酶阳性神经元的时序表达

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (6): 607-611.

扬子鳄大脑胚后发育中一氧化氮合酶阳性神经元的时序表达

郑兰荣¹, 汪仁平², 周永康², 吴孝兵³

¹皖南医学院病理解剖学教研室, 芜湖 241002, 安徽;
²安徽省扬子鳄繁殖研究中心, 宣城 242034, 安徽;
³安徽师范大学生命科学学院, 重要生物资源保护与利用安徽省重点实验室, 芜湖 241002, 安徽

收稿日期:2014-02-18 修回日期:2014-05-12 发布日期:2014-07-01
通讯作者: 吴孝兵, 男, 博士, 教授, 研究方向:分子系统学。Tel: 0553-3836873 E-mail: wuxb@ mail.ahnu.edu.cn
作者简介:郑兰荣, 女, 博士, 副教授, 研究方向:神经生物学。Tel: 13955382625 E-mail: zhenglanrong@qq.com
基金资助:
安徽省教育厅自然科学研究基金重点项目(KJ2014A263); 国家自然科学基金青年项目(31200828)

Sequential expression of nitirc oxide synthase positive neurons in post-embryonic development of cerebral cortex of the Chinese alligator

ZHENG Lan-rong¹, WANG Ren-ping², ZHOU Yong-kang², WU Xiao-bing³

¹Department of Pathology, Wannan Medical College, Wuhu 241002, Anhui, China;²Anhui Research Center for Chinese Alligator Reproduction, Xuancheng 242034, Anhui, China;
³Key Laboratory for Conservation and Exploitation of Biological Resource in Anhui Province, College of Life Sciences, Wuhu 241002, Anhui, China

Received:2014-02-18 Revised:2014-05-12 Published:2014-07-01

摘要/Abstract

摘要： 目的: 探讨一氧化氮合酶(NOS1)在扬子鳄大脑胚后发育中的时序表达。方法: 用免疫荧光细胞化学方法观察NOS1在0月、3月、6月、12月、24月和120月龄的扬子鳄大脑皮质中的定位表达。结果: 免疫荧光细胞化学显示, 0月期扬子鳄大脑皮质区分子层未见有荧光反应, 而外网状层、细胞层、内网状层均有NOS1阳性荧光反应, 随着年龄增长, 大脑皮质区增厚, 分子层有阳性荧光反应, 主要表现在细胞层中NOS1阳性荧光反应增强。到120月龄, NOS1阳性荧光在细胞层集中, 黄绿色明亮荧光增多, 并有荧光尾线伸出, 外网状层、内网状层均增厚。在各年龄期, 血管周围可见黄绿色荧光反应。通过对其表达的荧光密度图像分析得出各年龄段表达有统计学差异(P<0.05), 且随着年龄增长, 表达逐渐增强。结论: NOS1参与了扬子鳄大脑胚后生长发育。

关键词: 扬子鳄, 大脑, 胚后发育, 一氧化氮合酶, 免疫荧光细胞化学

Abstract: AIM : To investigate sequential changes of nitirc oxide synthase expression in thepostembryonic development of cerebral cortex of the Chinese alligator.METHODS: Immunofluorescent technique was used to detect the temporal expression concerning its variation at location and content of NOS1 in the cerebral cortex of the Chinese alligator by postembryonic age of 0 mh(newly hatched), 3 mh, 6 mh, 12 mh, 24 mh and 120 mhs. RESULTS: Immunofluorescent experiments showed that NOS1-positive fluorescence reaction was not seen at the molecular layer of cerebral cortex from the alligator aged 0 mh, but seen at the other 3 layers. With increased ages, the layers of cerebral cortex gradually thickened, fluorescence reaction in the molecular layer appeared with enhanced positive reaction in the cell layer. By 120 mhs, NOS1-positive reaction amassed in the cell layer, which yellowish green and bright fluorescence increased and tailed out, while fluorescence of the outer and inner plexiform layers thickened. Yellowish green fluorescence were seen around vessels at all ages.Through analysis to its corresponding fluorescence density image, the result showed that the expression of various ages were discrepant (P<0.05) and gradually enhanced with increased ages. CONCLUSION: NOS1 is involved in the post-embryonic development of cerebral cortex of the Chinese alligator.

Key words: Chinese alligator, cerebral cortex, postembryonic development, nitirc oxide synthase, immunofluorescence

中图分类号:

R965.2

郑兰荣, 汪仁平, 周永康, 吴孝兵. 扬子鳄大脑胚后发育中一氧化氮合酶阳性神经元的时序表达[J]. 中国临床药理学与治疗学, 2014, 19(6): 607-611.

ZHENG Lan-rong, WANG Ren-ping, ZHOU Yong-kang, WU Xiao-bing. Sequential expression of nitirc oxide synthase positive neurons in post-embryonic development of cerebral cortex of the Chinese alligator[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(6): 607-611.

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