关键词: n-3 多不饱和脂肪酸, 脑源性神经营养因子, 脑发育, 早期营养, 小鼠

Abstract: Objective To investigate the effects of n-3 polyunsaturated fatty acids (n-3 PUFAs) during maternal pregnancy and lactation on expression of brain-derived neurotrophic factor (bdnf) in offspring. Methods Forty 6~8 week-old C57BL/6J female mice were randomly divided into 5 groups,and fed with the n-3 PUFA deficient diet,and four n-3 PUFAs diets with different ratios of n-6/n-3 PUFAs at 15∶1,5∶1,1∶1 and 1∶5 respectively.Followed by 6 weeks feeding,the mice were mated for breeding pups,who received the same diets with their mothers.At postnatal 7,21 d and 3 months respectively,pups were sacrificed and the brain were used for detecting bdnf expression.Equal pups from groups of n-3 PUFA deficient diet and n-6/n-3 PUFAs (5∶1) diet were selected and diets were changed each other at postnatal 21 d lasting for 3 months.The mRNA expressions of bdnf in the brain cortex were measured by real-time RT-PCR. Results Compared to n-3 PUFAs deficient diet group,at both postnatal 7 d and 21 d,the increased bdnf mRNA expression was only seen in the n-6/n-3 PUFAs (1∶5) diet group;at 3 months,all the diets containing n-3 PUFAS had increased bdnf mRNA level.Feeding with the n-6/n-3 PUFAs (5∶1) diet to pups from the n-3 deficient diet group did not increase bdnf mRNA expression,whereas feeding with the n-3 PUFA deficient diet to pups from the n-6/n-3 PUFAs (5∶1) diet group increased bdnf expression. Conclusion Higher dietary intake of n-3 PUFAs during maternal pregnancy and lactation may be needed for optimal expression of bdnf gene.The appropriate n-3 PUFA intake may be beneficial in keeping bdnf expression normal in adult.

Key words: n-3 polyunsaturated fatty acids, brain-derived neurotrophic factor, brain development, early nutrition, mouse