支气管哮喘大鼠肺内c-fos蛋白表达和神经肽含量相关性研究

doi:10.11852/zgetbjzz2014-22-07-13

中国儿童保健杂志 ›› 2014, Vol. 22 ›› Issue (7): 709-712.DOI: 10.11852/zgetbjzz2014-22-07-13

支气管哮喘大鼠肺内c-fos蛋白表达和神经肽含量相关性研究

刘海燕¹, 侯伟¹, 杨旭东², 宗长红²

1 西安交通大学医学院第二附属医院儿科, 陕西西安 710004;
2 西安交通大学医学院遗传与分子生物学系, 陕西西安 710061

收稿日期:2013-09-11 发布日期:2014-07-10 出版日期:2014-07-10
作者简介:刘海燕(1971-), 女, 陕西人, 本科学历, 主要从事儿童呼吸系统疾病与儿童哮喘防治的研究。
基金资助:
陕西省科学技术研究发展计划项目[2007K-01(15)]

Correlation of distribution of c-fos and neuropetdides in lung of asthmatic rat.

LIU Hai-yan¹, HOU Wei¹, YANG Xu-dong², ZONG Chang-hong²

1 Department of Pediatrics, Second Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China;
2 Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China

Received:2013-09-11 Online:2014-07-10 Published:2014-07-10

摘要/Abstract

摘要： 目的探讨支气管哮喘大鼠肺内c-fos蛋白表达和神经肽P物质(substance P, SP)和血管活性肠肽(vasoactive intestinal peptide, VIP)含量及分析之间的相关性。方法 32只SD大鼠随机分成正常对照组、哮喘非急性发作期组、哮喘急性发作组和地塞米松干预组。采用免疫组织化学进行组织学观察, 采用酶联免疫吸附试验法测定肺泡灌洗液中SP和VIP的浓度。结果哮喘大鼠肺泡灌洗液中SP浓度较正常对照组显著增高(P＜0.000 1), VIP浓度显著降低(P＜0.000 1), 哮喘恢复组、哮喘急性期发作组及地塞米松干预组肺组织中c-fos蛋白光密度值与肺泡灌洗液SP浓度呈正相关(r分别为0.908、0.967、0.865, P<0.05或<0.001), 与肺泡灌洗液VIP浓度呈负相关(r分别为-0.974、-0.949、-0.962, P均≤0.001)。结论哮喘大鼠肺内c-fos蛋白表达、神经肽含量与哮喘发作有关。

关键词: 支气管哮喘, c-fos蛋白, 神经肽

Abstract: Objective To investigate the change of distribution of c-fos and neuropetides in the lung of asthmatic rat. Methods Thirty-two Wistar rats were divided into control group, asthmatic recover group, acute episode group, dexameth intervention group.Immunohistochemistry and computer image analysis were used to observe the c-fos change of the lung and the contents of substance P(SP) and vasoactive intestinal peptide(VIP) in lung tissues were detected by ELISA. Results The contents of SP in the BALF of asthmatic recover group, acute episode group, dexameth intervention group rats were significantly higher than that in control group(P＜0.000 1), and the contents of VIP were significantly lower.There were significantly positive correlations between the SP and the optical density value of c-fos(r=0.908, 0.967, 0.865, P<0.05 or <0.001).There were significantly negative correlations between VIP and optical density value of c-fos (r=-0.974, -0.949, -0.962, P all ≤0.001). Conclusion There is correlation between neuropeptides, c-fos proteinum and asthmatic attack asthmatic rats.

Key words: bronchial asthma, c-fos proteinum, neuropeptide

中图分类号:

R256.12

刘海燕, 侯伟, 杨旭东, 宗长红. 支气管哮喘大鼠肺内c-fos蛋白表达和神经肽含量相关性研究[J]. 中国儿童保健杂志, 2014, 22(7): 709-712.

LIU Hai-yan, HOU Wei, YANG Xu-dong, ZONG Chang-hong. Correlation of distribution of c-fos and neuropetdides in lung of asthmatic rat.[J]. journal1, 2014, 22(7): 709-712.

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支气管哮喘大鼠肺内c-fos蛋白表达和神经肽含量相关性研究

Correlation of distribution of c-fos and neuropetdides in lung of asthmatic rat.

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