亚低温对新生鼠缺氧缺血性脑损害不同脑区p16和Bcl-2表达的影响研究

中国儿童保健杂志 ›› 2011, Vol. 19 ›› Issue (5): 433-436.

亚低温对新生鼠缺氧缺血性脑损害不同脑区p16和Bcl-2表达的影响研究

郭亚乐¹,李占魁²,李瑞林¹,黄绍平¹, 周戬平¹

1 西安交通大学医学院第二附属医院儿科,陕西西安 710004;
2 陕西省妇幼保健院新生儿科,陕西西安 710003

收稿日期:2011-02-09 发布日期:2011-05-06 出版日期:2011-05-06
作者简介:郭亚乐(1975-),女,陕西人,主治医师,硕士学位,主要研究方向为儿科学神经专业

The study of influnce of hypothermia to the expression of P16 and Bcl-2 levels in different brain tissues of the rats with hypoxic-ischemic brain damage

GUO Ya-le¹, LI Zhan-kui², LI Rui-lin¹, HUANG Shao-ping¹, ZHOU Jian-ping¹

1 Department of Pediatrics, the Second Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710004,China;
2 The Neonatal Department of Shaanxi Maternal and Child Care Service Centre,Xi'an, Shaanxi 710003,China

Received:2011-02-09 Online:2011-05-06 Published:2011-05-06

摘要/Abstract

摘要： 【目的】探讨亚低温对新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage, HIBD)后脑灰质、室周白质的保护作用。【方法】建立新生大鼠HIBD模型,31 ℃、34 ℃亚低温全身干预3 h,观察缺氧缺血(hypoxic-ischemic, HI)后24 h、72 h、7 d脑皮质、海马、室周白质细胞凋亡、p16、bcl-2表达。【结果】 1)p16灰度:模型组表达高于假手术组,HI后24 h达高峰,31 ℃组表达减少,34 ℃组总表达不减少,但在HI后24 h表达减少,高峰延迟;2)凋亡细胞灰度:与p16结果一致;3)bcl-2灰度:也与p16结果一致,34 ℃组术后24 h、HI后72 h表达均减少;4)相关关系:p16、凋亡细胞、bcl-2灰度在各脑区表达一致,两两比较,有相关关系(P<0.01)。【结论】新生大鼠HIBD后亚低温干预通过降低p16、bcl-2的表达、减少细胞凋亡或延缓其高峰对脑灰质、室周白质组织起到保护作用。

关键词: 亚低温, 脑缺血, 白质损害, 凋亡

Abstract: 【Objective】 To investigate the protective effects of hypothermia to the grey matter and the periventricular white matter of the brains of the neonatal rats with hypoxic-ischemic brain damage(HIBD). 【Methods】 The 7d neonatal rats got the HIBD model. Hypothermias of 31 ℃, 34 ℃ were given to them for 3 h immediately after the operation. The degrees of grey of apoptosis, p16, bcl-2 of the cerebral cortex, the hippocampi tissue and the periventricular white matter in the brains of rats was used to observe when 24, 72, 168 h after the operation. 【Results】 1)The degrees of grey of p16: It was higher in the HIBD group than that in the pseuoperation group, the peak was at 24 hours after HI, it was deduced in 31 ℃ group, it was not deduced in 34 ℃group,but the peak was delated . 2) The degrees of grey of apoptosis: It was the same as that of p16. 3) The degrees of grey of bcl-2: It was the same as that of p16,it were deduced 24, 72 hours after HI in the 34 ℃ hypothermia group . 4) Correlation: there were the correlation ships (P<0.01) between either two of the degrees of the greys of p16, apoptosis, bcl-2 in all the brain tissues. 【Conclusions】 Hypothermia intervention could obviously deduce the express of p16, bcl-2, deduce apoptosis or delate their peaks of neonatal rats, brains with HIBD, so protected their grey matter tissue and the white matter tissue.

Key words: hypothermia, ischemic brain damage, white matter damage, apoptosis

中图分类号:

Q95-33

郭亚乐,李占魁,李瑞林,黄绍平, 周戬平. 亚低温对新生鼠缺氧缺血性脑损害不同脑区p16和Bcl-2表达的影响研究[J]. 中国儿童保健杂志, 2011, 19(5): 433-436.

GUO Ya-le, LI Zhan-kui, LI Rui-lin, HUANG Shao-ping, ZHOU Jian-ping. The study of influnce of hypothermia to the expression of P16 and Bcl-2 levels in different brain tissues of the rats with hypoxic-ischemic brain damage[J]. journal1, 2011, 19(5): 433-436.

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