P53基因多态性及环境因素与低出生体重儿的关系

中国儿童保健杂志 ›› 2013, Vol. 21 ›› Issue (2): 127-130.

P53基因多态性及环境因素与低出生体重儿的关系

吴少晶¹,刘文静¹,张华²,曾红²,王芸²,郑明慈¹

1 桂林医学院附属医院围产医学研究室,广西桂林 541001;
2 桂林医学院附属医院新生儿科,广西桂林 541001

收稿日期:2012-09-14 发布日期:2013-02-06 出版日期:2013-02-06
通讯作者: 郑明慈,E-mail:zhengmc_cn@163.com
作者简介:吴少晶(1985-),女,医师,硕士研究生,研究方向为营养性疾病。
基金资助:
中国疾病预防与控制中心达能营养中心膳食营养研究与宣教基金(DIC2009-12)

Relationships among p53 gene polymorphisms,environmental factors and low birth weight infants.

WU Shao-jing¹,LIU Wen-jing¹,ZHANG Hua²,ZENG Hong²,WANG Yun²,ZHENG Ming-ci¹.

1 Department of Perinatal Medicine,the Affiliated Hospital of Guilin Medical College,Guilin,Guangxi 541001,China;
2 Department of Neonatology,the Affiliated Hospital of Guilin Medical College,Guilin,Guangxi 541001,China

Received:2012-09-14 Online:2013-02-06 Published:2013-02-06

摘要/Abstract

摘要： 目的探讨新生儿P53基因多态性及环境因素与低出生体重儿之间是否存在关联。方法采用病例对照研究方法,收集母亲和新生儿资料,并根据新生儿出生体重和孕周进行分组,分为健康对照组和低出生体重组,其中低出生体重组包括早产儿组和小于胎龄儿组。使用多聚酶链-限制性片段长度多态性(PCR-RFLP)方法检测其外周血p53基因的2个多态性位点p53codon72、p53PIN3(rs1042522,rs17878362)并比较各自的基因型和等位基因的分布频率。结果 1)多因素非条件Logistic回归分析显示低出生体重儿的发生与胎儿性别、产妇文化程度、产前检查和居住地有关,回归系数分别为-0.710、1.055、0.825、-0.676。2)p53 codon72位点基因型总体分布差异有统计学意(χ²=19.182,P=0.001),而 p53PIN3位点的基因型总体分布差异没有统计学意义(χ²=0.566,P=0.754)。p53codon72位点Pro/Pro基因型在早产儿组和小于胎龄儿组的分布明显高于健康对组,其两组携带Pro/Pro基因型风险与健康对照组的OR值分别为2.317(95%CI:1.290~4.162,P=0.002)、2.805(95%CI:1.599~4.919,P=0.000)。结论遗传和环境因素及其交互作用在低出生体重儿的发生中均起着重要的作用。p53codon72位点多态性与低出生体重遗传易感性存在相关性,Pro等位基因可能是低出生体重的遗传易感基因。

关键词: P53基因多态性, 环境因素, PCR-RFLP, 低出生体重儿, 新生儿

Abstract: Objective To examine the relationships among p53 gene polymorphisms,environmental factors,and low birth weight infants. Methods A case-control study was conducted to collect the data of mothers and newborns,according to birth weight and gestational age,the infants were divided into healthy control group and low birth weight group (including preterm infants group and small for gestational age group).DNA from peripheral blood samples was examined by polymerase chain-restriction fragment length polymorphism (PCR-RFLP) method to detect the two p53 gene polymorphic situs p53codon72,p53PIN3 (rs1042522,rs17878362) and compared the genotype and allele frequency distribution in three groups. Results 1)Multivariate unconditional logistic regression analysis showed that the occurrence of low birth weight infants were associated with fetal gender,maternal education level,prenatal care and place of residence,and the regression coefficients were -0.710,1.055,0.825,-0.676,respectively.2)There was significant difference in the total distribution frequency of p53codon72 situs(χ²=19.182,P=0.001),but no association was detected between the low birth weight group and the healthy control group in p53PIN3 situs(χ²=0.566,P=0.754).The distribution frequencies of Pro/Pro genotype in p53codon72 situs in preterm infants group and small for gestational age group were significantly higher than those in normal birth weight infants group,individuals with the homozygous Pro/Pro genotype had increased risks of low birth weight[(OR=2.317,95%CI:1.290~4.162,P=0.002),(OR=2.805,95%CI:1.599~4.919,P=0.000)]. Conclusions Genetic and environmental factors and their interactions plays an important role in the incidence of low birth weight infants.p53codon72 situs polymorphism is related to low birth weight,and individuals allele of the p53 gene are at increased risk of low birth weight.

Key words: P53 gene polymorphism, environment factors, polymerase chainrestriction fragment length polymorphism, low birth weight infants, newborn

中图分类号:

R722

吴少晶,刘文静,张华,曾红,王芸,郑明慈. P53基因多态性及环境因素与低出生体重儿的关系[J]. 中国儿童保健杂志, 2013, 21(2): 127-130.

WU Shao-jing,LIU Wen-jing,ZHANG Hua,ZENG Hong,WANG Yun,ZHENG Ming-ci.. Relationships among p53 gene polymorphisms,environmental factors and low birth weight infants.[J]. journal1, 2013, 21(2): 127-130.

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