间断性低氧对幼鼠认知功能及海马突触超微结构的影响

中国儿童保健杂志 ›› 2013, Vol. 21 ›› Issue (6): 604-606.

间断性低氧对幼鼠认知功能及海马突触超微结构的影响

陈燕¹,赵春玲¹,余广¹,程基焱²

泸州医学院1 生理学教研室,2 电镜室,四川泸州 646000

收稿日期:2012-12-16 发布日期:2013-06-06 出版日期:2013-06-06
作者简介:陈燕(1979-),女,讲师,硕士研究生,主要研究方向为缺氧性脑损伤的发病机制和防治。

Effects of intermittent hypoxia on cognition and the ultrastructure of hippocampal synapses in immature rats.

CHEN Yan¹,ZHAO Chun-ling¹,YU Guang¹,CHENG Ji-yan².

1 Department of Physiology,2 Electron Microscopy Room,Luzhou Medical College,Luzhou,Sichan 646000,China

Received:2012-12-16 Online:2013-06-06 Published:2013-06-06

摘要/Abstract

摘要： 目的观察慢性间断性低氧(chronic intermittent hypoxia,CIH)对幼鼠认知功能及海马突触超微结构的进行性影响。方法雄性21日龄SD幼鼠66只,体重(50±5)g,随机分为正常对照组(unhandled control group,UC组)和慢性间断性低氧组(CIH组),每组33只,每组又分为2,4,6周3个时间点,每个时间点11只幼鼠。CIH组每日间断缺氧8 h,通过Morris水迷宫测试幼鼠学习和记忆能力,利用透射电镜观察海马CA1区突触超微结构的变化。结果 1)Morris水迷宫定位航行试验:CIH组幼鼠逃避潜伏期明显长于UC组,两组之间差异具有统计学意义(P<0.05)。2)Morris水迷宫空间搜索试验:与UC组比较,CIH组跨越目标象限时间占整个游泳时间的百分率明显减少,差异具有统计学意义(P<0.05)。与UC组比较,CIH组穿越平台次数明显减少,差异具有统计学意义(P<0.05)。3)电镜下可见CIH组突触小泡数量减少,结构不清,线粒体出现空泡样改变。UC组无明显病变。结论 CIH致幼鼠认知功能进行性下降与海马CA1区突触超微结构的改变密切相关。

关键词: 低氧, 认知, 海马, 超微结构, 突触, 幼鼠

Abstract: Objective To study the progressive effects of chronic intermittent hypoxia(CIH) on cognition and the ultrastructure of hippocampal synapses in immature rats. Methods A total of Sixty-six healthy,male,21-day-old Sprague-Dawley immature rats,weighing (50±5) g,was randomly averagely divided into two groups:unhandled control(UC)group and CIH group.Every group was divided into three time points:2,4 and 6 weeks(n=11).CIH-handled immature rats were exposed to intermittent hypoxia in designed cabin 8 hours every day.The cognitive function was assessed by the Morris Water Maze(MWM).The ultrastructure of hippocampal synapses were observed by electron microscope. Results 1)MWM of place navigation test:the escape latency in CIH immature rats was significantly longer compared with that in UC rats(P< 0.05);2)MWM of spatial probe test:the percentage of time spent on crossing the target quadrant to the total swimming time in CIH group was significantly decreased compared with that in UC group(P<0.05).The number of times of crossing the platform in CIH group was significantly reduced compared with that in UC group(P<0.05);3)Changes in the ultrastructure of hippocampal synapses were obvious in CIH group as compared with the UC group. Conclusion Chronic intermittent hypoxia induced slowly progressive cognition impairments in immature rats,which maybe associated with the changes in the ultrastructure of hippocampal synapses exposed to CIH.

Key words: hypoxia, cognition, hippocampus, ultrastructure, synapses, immature rats

中图分类号:

R749.94

陈燕,赵春玲,余广,程基焱. 间断性低氧对幼鼠认知功能及海马突触超微结构的影响[J]. 中国儿童保健杂志, 2013, 21(6): 604-606.

CHEN Yan,ZHAO Chun-ling,YU Guang,CHENG Ji-yan.. Effects of intermittent hypoxia on cognition and the ultrastructure of hippocampal synapses in immature rats.[J]. journal1, 2013, 21(6): 604-606.

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