丙酮酸乙酯对宫内感染致脑损伤新生仔鼠AQP4表达和脑组织超微结构的影响

doi:10.11852/zgetbjzz2018-26-11-09

中国儿童保健杂志 ›› 2018, Vol. 26 ›› Issue (11): 1194-1198.DOI: 10.11852/zgetbjzz2018-26-11-09

丙酮酸乙酯对宫内感染致脑损伤新生仔鼠AQP4表达和脑组织超微结构的影响

宋福祥,孔祥颖,庞伟,谭丽萍,郭津

佳木斯大学康复医学院,佳木斯大学附属第三医院,佳木斯大学儿童康复神经实验室,黑龙江佳木斯 154003

收稿日期:2018-05-14 修回日期:2018-06-11
通讯作者: 孔祥颖,E-mail:kxylindsay@163.com
作者简介:宋福祥(1981-),男,黑龙江人,讲师,硕士学位,主要研究方向为小儿脑损伤发病机制及早期防治研究
基金资助:
佳木斯大学科学技术研究项目(13Z1201559)

Effects of ethyl pyruvate on expression of aquaporin-4 and ultrastructure changes in neonatal rats with brain damage caused by intrauterine infection

SONG Fu-xiang, KONG Xiang-ying, PANG Wei, TAN Li-ping, GUO Jin

Rehabilitation Medicine of Jiamusi university,the Third Affiliated Hospital of Jiamusi university, Children Neural Rehabilitation Laboratory of Jiamusi University, Jiamusi, Heilongjiang 154003, China

Received:2018-05-14 Revised:2018-06-11
Contact: KONG Xiang-ying, E-mail:kxylindsay@163.com

摘要/Abstract

摘要： 目的研究丙酮酸乙酯(EP)对宫内感染致新生仔鼠脑损伤水通道蛋白4(AQP4)表达和脑组织超微结构的影响。方法选取36只孕鼠随机分为 LPS组(n=12)、EP组(n=12)、NS组(n=12)。LPS组:孕17 d、18 d连续两天腹腔注射 LPS 380 μg/kg;EP组:同法制备孕鼠宫内感染模型,并在注射LPS后立即给予孕鼠腹腔注射 EP 40 mg/kg;NS组:腹腔注射同等量生理盐水。对孕鼠分娩后的胎盘和新生仔鼠脑组织进行HE染色观察宫内感染和脑损伤情况。剔除早产鼠,各组随机选取新生仔鼠36只,于生后12、24、48 h对脑组织进行免疫组织化学染色,观察AQP4的表达;电镜观察脑组织超微结构的变化。结果与NS组比较,LPS组孕鼠胎盘组织可见大量的炎性细胞浸润,有宫内感染,LPS组仔鼠HE染色提示有脑损伤;与LPS组相比较,EP组仔鼠出生后24 h、48 h脑组织AQP4的表达量均低于LPS组(P<0.05);电镜下LPS组神经细胞超微结构显示损伤严重。结论丙酮酸乙酯对宫内感染致脑损伤新生仔鼠的脑组织具有一定的神经保护作用,可能与AQP4表达水平有关。

关键词: 丙酮酸乙酯, 宫内感染, 脑损伤, 水通道蛋白4, 脑超微结构

Abstract: Objective To study the effect of ethyl pyruvate (EP) in the expression of aquaporin-4(AQP4) and ultrastructure changes in neonatal rats with brain damage caused by intrauterine infection.Methods A total of 36 pregnant Wistar rats were enrolled in this study, and were divided into lipopolysaccharide (LPS) group, EP group and normal saline(NS) group, with 12 rats in each group. LPS group was consecutively injected with LPS (380 μg/(kg·d) intraperitoneally, EP group were consecutively injected with lipopolysaccharide intraperitoneally, and EP was administrated intraperitoneally at a dose of 40 mg/kg immediately. NS group was injected with normal saline intraperitoneally as control group. After the delivery of pups, thirty-six pups were selected. Placenta of rats were taken for hematoxylline-eosin (HE) staining to confirm its intrauterine infection in 12 hours, 24 hours,48 hours after birth. Aquaporin-4 expression in brain tissue was studied through immunohistochemical analysis. Ultrastructure changes in damaged brain and control group were studied electron-microscopically.Results Obvious pathological changes were observed in the placenta of LPS group. The expression level of AQP4 was lower in EP group and NS group in 24 hours and 48 hours after birth than that in LPS group (P<0.05). LPS group nerve cells ultrastructure showed serious injury.Conclusion Ethyl pyruvate has neuro-protective effect on LPS-induced brain injury, and may be associated with the expression level of AQP4.

Key words: ethyl pyruvate, intrauterine infection, brain injury, AQP4, ultrastructure

中图分类号:

R179

宋福祥,孔祥颖,庞伟,谭丽萍,郭津. 丙酮酸乙酯对宫内感染致脑损伤新生仔鼠AQP4表达和脑组织超微结构的影响[J]. 中国儿童保健杂志, 2018, 26(11): 1194-1198.

SONG Fu-xiang, KONG Xiang-ying, PANG Wei, TAN Li-ping, GUO Jin. Effects of ethyl pyruvate on expression of aquaporin-4 and ultrastructure changes in neonatal rats with brain damage caused by intrauterine infection[J]. journal1, 2018, 26(11): 1194-1198.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu41.magtech.com.cn/Jwk_zgetbjzz/CN/10.11852/zgetbjzz2018-26-11-09

https://manu41.magtech.com.cn/Jwk_zgetbjzz/CN/Y2018/V26/I11/1194

参考文献

[1] 李晓捷,唐久来,马丙祥,等.脑性瘫痪的定义、诊断标准及临床分型[J].中华实用儿科临床杂志,2014,29(19):1520.
[2] Boyle AK,Rinaldi SF,Norman JE,et al.Preterm birth:inflammation, fetal injury and treatment strategies[J].J Reprod Immunol,2017,119(2):62-66.
[3] MacLennan AH,Thompson SC,Gecz J.Cerebral palsy:causes, pathways, and the role of genetic variants [J].Am J Obstet Gynecol,2015,213(6):779-788.
[4] Burd I,Balakrishnan B,Kannan S.Models of fetal brain injury,intrauterine inflammation,and preterm birth [J].Am J Reprod Immunol,2012,67(4):287-294.
[5] Djedovic＇ N, Stanisavljevic S, Jevtic＇ B,et al. Anti-encephalitogenic effects of ethyl pyruvate are reflected in the central nervous system and the gut[J].Biomed Pharmacother,2017,96(12):78-85.
[6] Li W,Lou J,Wei L,et al.Ethyl pyruvate protects PC12 cells from oxygen-glucose deprivation:A potential role in ischemic cerebrovascular disease[J].Biomed Pharmacother,2017,92(8):168-174.
[7] 孔祥颖,李晓捷,宋福祥,等.丙酮酸乙酯对宫内感染致脑损伤新生仔鼠12-脂氧合酶表达和细胞凋亡的影响[J].中国儿童保健杂志,2015,23(8):817-820.
[8] Wang WW,Xie CL,Zhou LL,et al. The function of aquaporin4 in ischemic brain edema[J].Clin Neurol Neurosurg,2014,127(12):5-9.
[9] Heling Chu,Chuyi Huang,Hongyan Ding,et al. Aquaporin-4 and cerebrovascular diseases[J].Int J Mol Sci,2016,17(8):1249.
[10] Zhao J,Chen Y,Xu Y,et al. Effect of intrauterine infection on brain development and injury[J].Int J Dev Neurosci, 2013,31(7):543-549.
[11] 李晓捷,高晶,孙忠人.宫内感染致早产鼠脑瘫动物模型制备及其鉴定的实验研究[J].中国康复医学杂志,2004,12(1):61-64.
[12] Yin J,Zhang H,Chen H,et al. Hypertonic saline alleviates brain edema after traumatic brain injury via downregulation of aquaporin 4 in rats[J].Med Sci Monit,2018,24(3):1863-1870.
[13] Nakano T,Nishigami C,Irie K,et al.Goreisan prevents brain edema after cerebral ischemic stroke by inhibiting aquaporin 4 upregulation in mice[J].J Stroke Cerebrovasc Dis,2018,27(3):758-763.
[14] Wang C,Yan M,Jiang H,et al. Mechanism of aquaporin 4 (AQP4) up-regulation in rat cerebral edema under hypobaric hypoxia and the preventative effect of puerarin[J].Life Sci,2018,193(1):270-281.
[15] Gandham EJ, Vasudevan P, Moorthy RK,et al.Cortical Aquaporin-4 in relation to brain oedema and neurological function of cortical cryo-injured mice[J].J Clin Neurosci,2017,44(10):294-299.
[16] Lan YL,Chen JJ,Hu G,et al. Aquaporin 4 in astrocytes is a target for therapy in alzheimer′s disease[J].Curr Pharm Des,2017,23(33):4948-4957.
[17] 熊翱,金戈,熊仁平,等.沉默水通道蛋白4对脑创伤致大鼠学习记忆功能障碍的作用研究[J].中华危重病急救医学,2018,30(2):170-175.
[18] Kayiran O,Cuzdan SS,Uysal A,et al. Ethyl pyruvate improves skin flap survival after ischaemia reperfusion injury[J].Indian J Med Res,2017,146(3):369-374.
[19] Relja B,Wagner N,Franz N,et al.Ethyl pyruvate reduces acute lung damage following trauma and hemorrhagic shock via inhibition of NF-κB and HMGB1[J].Immunobiology.2018,223(3):310-318.
[20] Jung SM,Lee J, Baek SY,et al. Ethyl pyruvate ameliorates inflammatory arthritis in mice[J].Int Immunopharmacol.2017,52(11):333-341.
[21] Chen W,Lian J,Ye JJ,et al. Ethyl pyruvate reverses development of pseudomonas aeruginosa pneumonia during sepsis-induced immunosuppression[J].Int Immunopharmacol.2017,52(11):61-69.
[22] Zhang M, Hu X, Li S,et al.Hepatoprotective effects of ethyl pyruvate against CCl4-induced hepatic fibrosis via inhibition of TLR4/NF-κB signaling and up-regulation of MMPs/TIMPs ratio[J].Clin Res Hepatol Gastroenterol,2018,42(1):72-81.