关键词: 肥胖, 儿童, 细胞死亡激活剂-DNA断裂因子相似蛋白C, 胰岛素抵抗, 肿瘤坏死因子-α

Abstract: Objective To evaluate the expression level of cell death-inducingDNA fragmentation factor45-like effector C(CIDEC) in adipose tissue of obese children and its correlation with lipolysis and insulin resistance. Methods A total of 42 children who underwent surgery for non-inflammatory diseases such as hernia and cryptomchidism in the Second Affiliated Hospital of Xi′an Jiaotong University and Xi ′an Children′s Hospital were selected in this study from July 2014 to September 2016,and were divided into obesity group and control group.The body mass index (BMI),waist circumference,blood pressure,blood lipids,fasting blood glucose,serum free fatty acid (NEFA) and insulin resistance index (HOMA-IR) of children were measured.Meanwhile,western blot and RT-PCR were applied to detect the expression level of CIDEC,tumor necrosis factor-α(TNF-α),peroxisome proliferators-activated receptors (PPARγ).And the associations among TNF-α,NEFA,HOMA-IR and CIDEC were studied by Pearson correlation analysis. Results 1) Indexes in obese children,including BMI,waist circumference,systolic blood pressure (SBP),diastolic blood pressure (DBP),HOMA-IR and NEFA,were significantly higher than those in normal controls(P＜0.05),while HDL-C was relatively lower in obese group.2) The expression level of CIDEC mRNA and protein in adipose tissue of obese group decreased (P<0.01),while the expression of TNF-α mRNA increased (P<0.01).3) Correlation analysis showed that the expression level of CIDEC mRNA in adipose tissue was negatively correlated with the levels of TNF-α mRNA,HOMA-IR,NEFA (r=-0.583,-0.560,-0.606,P＜0.05). Conclusions TNF-α causes adipocyte lipolysis,thereby leading to insulin resistance,possibly resulting from down-regulating the expression of CIDEC.

Key words: obesity, children, cell death-inducing DNA fragmentation factor 45-like effector C, insulin resistance, tumor necrosis factor-α