关键词: 肠道菌群, 孤独症谱系障碍, 多样性, 儿童, 高通量测序

Abstract: Objective To analyze the structure and diversity of intestinal flora in children with autism spectrum disorder (ASD). Methods The number of 20 children with ASD diagnosed in Maternal Child Health Hospital of Guangxi Autonomous Region and the number of 20 healthy children from January to June 2018 were selected as the subjects in the study.Total DNA was extracted from feces of two groups of children,and V4/V5 region of 16S rDNA was amplified for high throughput sequencing.The intestinal flora diversity of ASD children was analyzed by sequencing results. Results There were no significant differences in the coverage of sample libraries (Z=242.500,P=0.256),abundance index Chao1 (Z=250.000,P=0.181),ACE (Z=234.000,P=0.365),Shannon (Z=259.000,P=0.114) and Simpson (Z=146.000,P=0.145) indices between the two groups (P>0.05).At the gate level,there was no significant difference between the two groups in 8 species of bacteria (P>0.05),but at the generic level,there was significant difference between the two groups in 20 genera (P<0.05). Conclusions There are differences in intestinal flora between ASD children and healthy children.In clinic,the intestinal flora of ASD children can be accurately adjusted according to the characteristics of different bacteria genus to maintain the intestinal microecological balance and alleviate the gastrointestinal symptoms of ASD children.

Key words: intestinal flora, autism spectrum disorder, diversity, children, high throughput sequencing