关键词: 焦虑, 瘦素, 高架十字迷宫, 慢性束缚应激

Abstract: Objective To explore the effect of leptin treatment on anxiety-like behavior caused by chronic restraint stress in adolescent mice,in order to provide data for the treatment of adolescent emotional disorders. Methods Totally 50 mice aged 21-30 days were subjected to low-intensity (2 h) / high-intensity (4 h) restraint stress for 10 consecutive days.Anxiety-like behavior was observed by elevated plus maze (EPM) and open field test (OFT).Serum leptin level was measured by enzyme-linked immunosorbent assay. Results Serum leptin level in the high-intensity restraint stress (HCRS) group[(4.2±1.3) pg/ml]was lower than that in the control group[(10.4±2.9)pg/ml,P<0.05].In the EPM experiment,the open arm times and the open arm entries in the HCRS group were (68.5±18.9)s and 1.7±1.1,respectively,significantly less than those in the normal group[(101.3±19.8) s,3.8±1.8,P<0.01).After the treatment of leptin,the above two indexes increased to (93.8±16.4)s and 3.3±1.6,respectively,which were close to the normal level.In the OFT experiment,the time spent in central area of HCRS mice[(63.4±15.9)s]was lower than that of the control group[(142.5±20.2) s,P<0.01],and this index was prolonged to (130.5±26.5)s after the application of leptin.However,there was no significant change in the indexes of the adolescent mice experienced low-intensity restraint stress in the two experiments. Conclusion HCRS can cause the anxiety disorder of adolescentmice,and using chronic leptin increases serum leptin levels and improve anxiety symptom.

Key words: anxiety, leptin, elevated plus maze, chronic restraint stress