关键词: 维生素A, 1型糖尿病, 辅助性T淋巴细胞, 儿童

Abstract: 【Objective】 To explore change of serum concentrtion of vitamin A,and its influence on the balance of Th1/Th2 in children with type 1 diabetes mellitus. 【Methods】 Totally 32 children with T1DM were taken as T1DM group,and 28 healthy children as control group.The serum level of vitamin A was determined by the method of micro-flourescence,and the levels of IFN-γ and IL-4 in the supernatant were determined by enzyme-linked immunosorbent assay. 【Results】 1)The level of vitamin A in T1DM group was lower than that in control group(P<0.01).2)IFN-γ level produced from PBMC in T1DM group was higher than that in normal control group(P<0.05),IFN-γ/IL-4 ratio was higher than that of normal control group(P<0.01).3)The levels of vitamin A in T1DM group and normal control group both had negative correlation with the levels of IFN-γ produced from PBMC(P<0.05 or <0.01),and negative correlation with the ratio of IFN-γ/IL-4(P<0.05 or <0.01),but had no correlation with the levels of IL-4(P>0.05). 【Conclusions】 The deficiency of vitamin A is present in children with type 1 diabetes mellitus,which is closely correlated with the balance of Th1/Th2.

Key words: vitamin A, type 1 diabetes mellitus, helper-inducer T-lymphocytes, children