NYGGF4(PID1)基因过表达对脂肪细胞线粒体代谢的影响

中国儿童保健杂志 ›› 2012, Vol. 20 ›› Issue (11): 998-1000.

NYGGF4(PID1)基因过表达对脂肪细胞线粒体代谢的影响

史春梅^1,2,季晨博^1,2,张春梅^1,2,朱金改^1,2,陈玲^1,2,郭锡熔^1,2

1 南京医科大学附属南京妇幼保健院儿童保健科,江苏南京 210004;
2 南京医科大学儿科研究所,江苏南京 210029

收稿日期:2012-04-11 发布日期:2012-11-06 出版日期:2012-11-06
通讯作者: 郭锡熔,E-mail:xrguo@njmu.edu.cn
作者简介:史春梅(1987-),女,博士在读,研究方向为儿童肥胖症
基金资助:
国家自然科学基金(30973231,30571978);江苏省医学创新团队(LJ201108);江苏省六大人才高峰(卫生-39)

Influence of NYGGF4 (PID1) gene over-expression on mitochondrial metabolism in adipocytes

SHI Chun-mei^1,2,JI Chen-bo^1,2,ZHANG Chun-mei^1,2,ZHU Jin-gai^1,2,CHEN Ling^1,2,GUO Xi-rong^1,2

1 Nanjing Maternal and Child Health Care Hospital Affiliated to Nanjing Medical University,Nanjing,Jiangsu 210004,China;
2 Institute of Pediatrics,Nanjing Medical University,Nanjing,Jiangsu 210029,China

Received:2012-04-11 Online:2012-11-06 Published:2012-11-06

摘要/Abstract

摘要： 【目的】观察NYGGF4(PID1)基因过表达对脂肪细胞线粒体代谢的影响。【方法】以3T3-L1前体脂肪细胞为研究对象,体外培养稳定转染NYGGF4(PID1)基因(NYGGF4-pcDNA3.1 (+)/myc-His B)的3T3-L1前体脂肪细胞,以转染空载体[pcDNA3.1(+)/myc-His B]的3T3-L1前体脂肪细胞为对照,经1-甲基-3异丁基黄嘌呤(MIX)加地塞米松、胰岛素方案诱导分化为成熟脂肪细胞,采用Real-time PCR检测线粒体代谢酶指标:己糖激酶(Hexokinase,HKI)、乙酰辅酶A羧化酶(Acetyl-CoA,ACC)、柠檬酸合成酶( citrate synthase,CS)、肉毒碱棕榈酰转移酶1(Carnitine palmitoyl-transferase 1,CPT1)、细胞色素C(Cytochrome c,Cyc-c)基因表达水平。【结果】 NYGGF4(PID1)过表达显著降低3T3-L1脂肪细胞己糖激酶、乙酰辅酶A羧化酶、柠檬酸合成酶、肉毒碱棕榈酰转移酶1、细胞色素C基因表达水平,差异有统计学意义。【结论】 NYGGF4(PID1)基因过表达,下调3T3-L1脂肪细胞中定位于线粒体的代谢关键酶,提示NYGGF4(PID1)基因过表达能影响3T3-L1脂肪细胞的线粒体代谢。

关键词: NYGGF4基因, 线粒体, 线粒体代谢, 3T3-L1脂肪细胞

Abstract: 【Objective】 To explore the effect of NYGGF4 (PID1) gene over-expression on mitochondrial metabolism in adipocytes. 【Methods】 3T3-L1 preadipocytes transfected with either a NYGGF4 expression vector or an empty expression vector were induced to differentiate into adipocytes.The expression levers of metabolic enzymes in mitochondria-Hexokinase (HKI),Acetyl-CoA (ACC),citrate synthase (CS),Carnitine palmitoyl-transferase 1 (CPT1),Cytochrome C (Cyc-C) were detected by Real-time PCR. 【Result】 NYGGF4 over-expression was significantly lower level of metabolic enzymes in mitochondria-HKI,ACC,CS,CPT1,Cyc-c in adipocytes than control (NC) cells. 【Conclusion】 In adipocytes with NYGGF4 gene over-expression,mitochondria metabolic enzymes mRNA is down- regulated.It could implicate that mitochondrial metablism is influenced in adipocytes with NYGGF4 gene over-expression.

Key words: NYGGF4 gene, mitochondria, mitochondrial metabolism, 3T3-L1 adipocytes

中图分类号:

Q786

史春梅,季晨博,张春梅,朱金改,陈玲,郭锡熔. NYGGF4(PID1)基因过表达对脂肪细胞线粒体代谢的影响[J]. 中国儿童保健杂志, 2012, 20(11): 998-1000.

SHI Chun-mei,JI Chen-bo,ZHANG Chun-mei,ZHU Jin-gai,CHEN Ling,GUO Xi-rong. Influence of NYGGF4 (PID1) gene over-expression on mitochondrial metabolism in adipocytes[J]. journal1, 2012, 20(11): 998-1000.

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