蟾毒灵对食管癌细胞株Kyse150放疗敏感性影响的研究

doi:10.12092/j.issn.1009-2501.2018.07.002

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (7): 728-733.doi: 10.12092/j.issn.1009-2501.2018.07.002

蟾毒灵对食管癌细胞株Kyse150放疗敏感性影响的研究

方为扬¹，李越¹，盛蕾¹，崔珍珍²，仲飞³，童铸廷¹

¹安徽医科大学第一附属医院肿瘤放疗科，²安徽医科大学医学院，³安徽医科大学第一附属医院肿瘤内科，合肥 230022，安徽

收稿日期:2018-04-09 修回日期:2018-05-07 出版日期:2018-07-26 发布日期:2018-07-20
通讯作者: 童铸廷，博士，副教授，硕士生导师，研究方向：肿瘤放射生物学。 Tel：055162923541 E-mail: 593077136@qq.com
作者简介:方为扬，女，硕士研究生，研究方向：肿瘤放射生物学。 Tel：15256926878 E-mail：846382564@qq.com
基金资助:
国家自然科学基金(81201743)；中国教育部博士基金(20123420120008)；安徽省博士后科学基金资助项目(2015B053)；安徽省高等学校省级质量工程大学生创新训练项目（201510366065）

Effects of bufalin on radiosensitivity of esophageal cancer Kyse150 cell line

FANG Weiyang¹, LI Yue¹, SHENG Lei¹, CUI Zhenzhen², ZHONG Fei³, TONG Zhuting¹

¹Department of Oncological Radiotherapy, the First Affiliated Hospital of Anhui Medical University; ² Anhui Medical University; ³Department of Oncology, the First Affiliated Hospital of Anhui Medical University, HeFei 230032, Anhui, China

Received:2018-04-09 Revised:2018-05-07 Online:2018-07-26 Published:2018-07-20

摘要/Abstract

摘要：

目的: 研究蟾毒灵对食管癌Kyse150细胞株放疗敏感性的影响。方法: 使用CCK-8方法测定Kyse150细胞株中蟾毒灵的IC₅₀值。通过克隆形成实验结果拟合L-Q曲线，求得细胞存活分数（SF2）和放疗增敏比（SER）。并利用Annexin V-FITC/PI双染法和Western blot测定蟾毒灵对放射诱导食管癌细胞株Kyse150凋亡的影响。最后通过克隆形成实验检测不同剂量蟾毒灵对放疗敏感性影响的变化。结果: CCK-8检测结果显示蟾毒灵在Kyse150细胞株中的IC₅₀值为129.1 nmol/L（95% CI:105.2-158.6）。通过克隆形成实验拟合的L-Q曲线显示蟾毒灵可显著降低放疗后细胞的克隆形成率，且蟾毒灵对Kyse150放疗增敏比（SER）=1.463。Annexin V-FITC/PI双染法和Western blot结果显示蟾毒灵可提高射线诱导的细胞凋亡，并显示蟾毒灵对食管癌细胞株Kyse150放疗增敏作用呈剂量依赖性。结论: 蟾毒灵在食管癌Kyse150细胞株中具有放疗增敏作用，且该作用为剂量依赖性。

关键词: 蟾毒灵, 食管癌, 放疗敏感性, 线性二次模型, 放疗增敏比

Abstract:

AIM: To investigate the radio-sensitivity effect of bufalin in Kyse150 cell line. METHODS: CCK-8 method was used to determine the IC₅₀ value of bufalin in Kyse150 cell line. The linear-quadratic (L-Q) curve was fitted according to the results of cloning formation and calculated the survival fraction of 2Gy (SF2) and sensitization enhancement ratio (SER). Annexin V-FITC/PI double staining and Western blot were used to detect the effect of bufalin on apoptosis of esophageal cancer Kyse150 cell line. The effect of different bufalin doses on Kyse150 cell line radiosenstivity was detected by cloning formation assay. RESULTS: CCK-8 results showed that the IC₅₀ value of bufalin in the Kyse150 cell line was 129.1 nmol/L (95% CI: 105.2-158.6). The L-Q curve fitted by colony formation assay showed that bufalin can significantly reduce the colony forming efficiency of cells after radiotherapy, and the sensitization enhancement ratio of bufalin in Kyse150 cell line was 1.463. Annexin V-FITC/PI double staining and Western blot results showed that bufalin can improve radiation-induced apoptosis, and showed that bufalin has a dose-dependent effect on radiosensitization of esophageal cancer Kyse150 cell line. CONCLUSION:Bufalin can enhance the radiosensitivity effect of the Kyse150 cell line in a dose-dependent manner.

Key words: bufalin, esophageal cancer, radiosensitivity, linear quadratic model, sensitization enhancement ratio

中图分类号:

R73
R965.2

方为扬，李越，盛蕾，崔珍珍，仲飞，童铸廷. 蟾毒灵对食管癌细胞株Kyse150放疗敏感性影响的研究[J]. 中国临床药理学与治疗学, 2018, 23(7): 728-733.

FANG Weiyang, LI Yue, SHENG Lei, CUI Zhenzhen, ZHONG Fei, TONG Zhuting. Effects of bufalin on radiosensitivity of esophageal cancer Kyse150 cell line[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(7): 728-733.

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蟾毒灵对食管癌细胞株Kyse150放疗敏感性影响的研究

Effects of bufalin on radiosensitivity of esophageal cancer Kyse150 cell line

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